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重要文件

14-845-M

Sigma-Aldrich

PIP5K1γ Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant human PIP5K1γ, full length. For use in Kinase Assays.

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.41

生物源

human

品質等級

重組細胞

expressed in baculovirus infected Sf21 cells

分子量

Mw 77 kDa

製造商/商標名

Upstate®

技術

activity assay: suitable (kinase)

NCBI登錄號

UniProt登錄號

基因資訊

human ... PIP5K1C(23396)

一般說明

N-terminal 6His-tagged, recombinant human PIP5K1γ, full length.
Product Source: Expressed by baculovirus in Sf21 insect cells

應用

Active, N-terminal 6His-tagged, recombinant human PIP5K1γ, full length. For use in Kinase Assays.
Research Category
Signaling
Research Sub Category
Oncology

Inflammation & Autoimmune Mechanisms

Diabetes

Alzheimer′s Disease

Neurodegenerative Diseases

生化/生理作用

Protein Target: PIP5K1γ
Target Sub-Family: PIPK

包裝

Also available in 250μg size as catalog # 14-845M - Call for pricing and availability.

品質

Routinely evaluated in the PIProfiler.

外觀

Immobilized metal affinity chromatography (IMAC)
10µg of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.

儲存和穩定性

6 months at -70°C

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

分析證明 (COA)

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Brittany D Wright et al.
Journal of biomolecular screening, 20(5), 655-662 (2014-12-24)
Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) regulate a variety of cellular processes, including signaling through G protein-coupled receptors (GPCRs), endocytosis, exocytosis, and cell migration. These lipid kinases synthesize phosphatidylinositol 4,5-bisphosphate (PIP2) from phosphatidylinositol 4-phosphate [PI(4)P]. Because small-molecule inhibitors of these lipid kinases

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