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重要文件

14-448-M

Sigma-Aldrich

Cdk2/Cyclin A Protein, active, 10 µg

Active, C-terminal His6-tagged human full length Cdk2 & N-terminal GST-tagged human full length Cyclin A, for use in Kinase Assays.

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.41

生物源

human

品質等級

重組細胞

expressed in baculovirus infected Sf21 cells

產品線

Upstate®

比活性

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

分子量

Mw 35 kDa

純化經由

(The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose)

物種活性

human

製造商/商標名

Upstate®

技術

activity assay: suitable (kinase)

登錄號

 (FUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. )
NM_001798.2

UniProt登錄號

一般說明

C-terminal His6-tagged human full length cdk2, and N-terminal GST-tagged human full length cyclin A

生化/生理作用

Protein Target: CDK2/cyclinA
Target Sub-Family: CMGC

包裝

Also available in 10 μg size and in bulk
Also available in 250μg size, please inquire for pricing and availability.

品質

routinely evaluated by phosphorylation of Histone H1

標靶描述

CDK2/cyclinA

聯結

Replaces: 14-407

外觀

The cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose

儲存和穩定性

6 months at -70°C

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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象形圖

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訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°C)

Not applicable


分析證明 (COA)

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