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Merck
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重要文件

14-415

Sigma-Aldrich

Ral Assay Reagent (Ral BP1, agarose)

同義詞:

Agarose Ral Assay, Ral BP1 Assay Reagent, Ral Detection Reagent

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About This Item

分類程式碼代碼:
12352204
eCl@ss:
32160405
NACRES:
NA.41

形狀

liquid

品質等級

製造商/商標名

Upstate®

技術

affinity binding assay: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

一般說明

GST fusion-protein, corresponding to residues 397-519 of rat Ral binding protein (RalBP1) bound to agarose. Also referred to as RLIP and RLP; specifically binds to and precipitates RalA-GTP and RalB-GTP from cell lysates.
Product Source: Expressed in E. coli.

品質

Routinely evaluated by Affinity Precipitation/Immunoblot Analysis

外觀

300 μg of protein in 600 μL of a 50% agarose slurry in 50 mM Tris, pH 7.5, 200 mM NaCl, 1% Triton X-100, 20 μM PMSF, 1 mM aprotinin prior to the addition of glycerol to 50%. Liquid suspension.

儲存和穩定性

1 year at -20°C

其他說明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2


分析證明 (COA)

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T Goi et al.
Molecular and cellular biology, 19(3), 1731-1741 (1999-02-18)
Ras proteins can activate at least three classes of downstream target proteins: Raf kinases, phosphatidylinositol-3 phosphate (PI3) kinase, and Ral-specific guanine nucleotide exchange factors (Ral-GEFs). In NIH 3T3 cells, activated Ral-GEFs contribute to Ras-induced cell proliferation and oncogenic transformation by

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