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Merck
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重要文件

14-392

Millipore

绿色荧光蛋白

同義詞:

GFP

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About This Item

分類程式碼代碼:
12352200
eCl@ss:
32160405
NACRES:
NA.32

重組細胞

expressed in E. coli

品質等級

化驗

>70%

形狀

liquid

分子量

Mw ~28 kDa

包裝

pkg of 300 μg

製造商/商標名

Upstate®

技術

western blot: suitable

GenBank登錄號

UniProt登錄號

運輸包裝

dry ice

一般說明

对应于全长绿色荧光蛋白的N末端带有HIS标签的融合蛋白,在大肠埃希菌中表达。绿色荧光蛋白(GFP)是维多利亚多管发光水母(Aequorea Victoria)中的一种细胞质蛋白。

生化/生理作用

绿色荧光蛋白(GFP)可以通过遗传改造,跟许多种蛋白形成融合蛋白,同时保持目标蛋白的活性和GFP的荧光。GFP可用作基因表达的报告基因,也可作为活细胞蛋白质定位研究的融合标签。还作为光学和显微探针,分析各种系统的生物功能。GFP非常稳定,适合作为体外研究工具使用。

品質

常规评估为蛋白凝胶和蛋白质免疫印迹的标准品,用于校准荧光计以及显微注射到细胞和组织中。

外觀

含20%甘油的PBS。

儲存和穩定性

在-20°C下保存1年

法律資訊

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Sebastian Strauss et al.
Nature methods, 15(9), 685-688 (2018-08-22)
Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small
Sonit Kumar Gogoi et al.
Langmuir : the ACS journal of surfaces and colloids, 22(22), 9322-9328 (2006-10-18)
Recombinant Escherichia coli (E. coli) bacteria expressing green fluorescent protein (GFP) was used as a model system to investigate the antimicrobial activities of Ag nanoparticles (NPs). A convenient in situ method of Ag NP synthesis using sodium borohydride, in the
Vincent Jung et al.
Proteomics, 22(9), e2100031-e2100031 (2021-12-28)
Biolayer interferometry (BLI) is a technology which allows to study the affinity between two interacting macro-molecules and to visualize their kinetic of interaction in real time. In this work, we combine BLI interaction measurement with mass spectrometry in order to
J L Casey et al.
Protein engineering, 13(6), 445-452 (2000-07-06)
We produced a fluorescent antibody as a single recombinant protein in Escherichia coli by fusing a red-shifted mutant of green fluorescent protein (EGFP) to a single-chain antibody variable fragment (scFv) specific for hepatitis B surface antigen (HepBsAg). GFP is a
Elisa M York et al.
Biomedical optics express, 10(9), 4381-4394 (2019-10-01)
Autofluorescence of endogenous molecules can provide valuable insights in both basic research and clinical applications. One such technique is fluorescence lifetime imaging (FLIM) of NAD(P)H, which serves as a correlate of glycolysis and electron transport chain rates in metabolically active

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