124020

Akt抑制剂X

The Akt Inhibitor X, also referenced under CAS 925681-41-0, controls the biological activity of Akt. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• 同義詞: Akt抑制剂X, 10-（4ʹ-（N-二乙氨基）丁基）-2-氯吩恶嗪，HCl，10-NCP
• 經驗公式（希爾表示法）: C₂₀H₂₅ClN₂O · xHCl
• CAS號碼: 925681-41-0
• 分子量：: 344.88 (free base basis)
• MDL號碼: MFCD09038570
• 分類程式碼代碼: 12352200
• NACRES: NA.77

屬性

• 品質等級: 100
• 化驗: ≥95% (HPLC)
• 形狀: solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic); protect from light
• 顏色: white
• 溶解度: water: 1 mg/mL
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• SMILES 字串: [Cl-].Clc1cc2c(cc1)Oc3c(cccc3)N2CCCC[N+H](CC)CC
• InChI: 1S/C20H25ClN2O.ClH/c1-3-22(4-2)13-7-8-14-23-17-9-5-6-10-19(17)24-20-12-11-16(21)15-18(20)23;/h5-6,9-12,15H,3-4,7-8,13-14H2,1-2H3;1H
• InChI 密鑰: SVKSJUIYYCQZEC-UHFFFAOYSA-N

描述

一般說明

Akt磷酸化及其体外激酶活性（完全抑制<5 M) with minimal effect on PI 3-K, PDK1, or SGK1. Shown to suppress growth of Rh (rhabdomyosarcoma) cell lines (IC₅₀ = 2-5 M), inhibit IGF-I-stimulated nuclear translocation of Akt, and prevent phosphorylation of the downstream targets, mTOR, p70S6 kinase, and S6 ribosomal protein. Unlike Akti1/2 (Cat. No. 124018), the mode of inhibition is not PH domain-dependent. Also shown to induce neuronal autophagy in an Akt- and mTOR-independent manner and enhances the clearance of misfolded protein. Also available as a 20 mM solution in H₂O(Cat. No. 124039 ）的细胞可渗透性、可逆性和选择性抑制剂。

生化／生理作用

主靶
Akt

二级靶标
Rn细胞系（IC₅₀ = 2-5 µM）

产物不与ATP竞争。

可逆性：是

细胞可渗透性：是

靶标IC₅₀：<5 µM 抗Akt；2-5 µM 抗Rh（横纹肌肉瘤）细胞系生长

包裝

用惰性气体包装

警告

毒性：刺激性（B）

重構

在复溶后分装并冻存（-20°C）。储备溶液在-20°C下可稳定保存至多3个月。

其他說明

Tsvetkov, A.S., et al. 2010.Proc.Natl.Acad.Sci. USAin press.
Thimmaiah, K.N., et al. 2005.J. Biol. Chem.280, 31924.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable