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Merck
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文件

12-309

Sigma-Aldrich

HeLa Nuclear Extract

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About This Item

分類程式碼代碼:
41105330
eCl@ss:
32011807
NACRES:
NA.41

生物源

human

品質等級

形狀

liquid

製造商/商標名

Upstate®

濃度

2.0 mg/mL

技術

western blot: suitable

運輸包裝

dry ice

一般說明

Nuclear extract prepared from human HeLa cells using a modified protocol of Dignam, et al., which contains a variety of DNA binding proteins and transcription factors.

細胞系描述

HeLa

品質

Routinely evaluated as a positive control for many of Upstates antibodies.

外觀

RIPA sample buffer containing inhibitors. Frozen solution.

儲存和穩定性

Stable for 6 months at -70°C from date of shipment.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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J D Dignam et al.
Nucleic acids research, 11(5), 1475-1489 (1983-03-11)
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major
Tabetha Sundin et al.
Biochimie, 94(12), 2639-2648 (2012-08-21)
Isoprenoids are recognized for their ability to suppress carcinogenic processes in vivo and in vitro. We previously established that the isoprenoid, perillyl alcohol, acted mechanistically on translation of specific proteins through modulation of mechanistic target of rapamycin (mTOR) signaling. Telomerase-the

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