1.09261

Lugol′s solution (diluted iodine-potassium iodide solution)

for the Gram staining method

• 同義詞: Iodine-potassium iodide solution
• 分類程式碼代碼: 41116130
• NACRES: NA.74

屬性

• 品質等級: 400
• 形狀: liquid
• IVD: for in vitro diagnostic use
• 技術: microbe id | staining: suitable
• pH值: 3.5 (20 °C in H₂O)
• bp: 100 °C/1013 hPa
• 密度: 1.01 g/cm³ at 20 °C
• 應用: clinical testing; diagnostic assay manufacturing; hematology; histology
• 儲存溫度: 15-25°C

描述

一般說明

Lugol′s solution (diluted iodine-potassium iodide solution - for the Gram staining method, is a ready-to-use solution used in Bacteriology, for example, for the staining of Gram bacteria. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet (Product number 1.09218), an aniline dye. After the treatment with iodine solution (Lugol′s solution), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, Product number 1.10218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Product number 1.09217) and will then appear pink to red.
Lugol′s solution (diluted iodine-potassium iodide solution) is capable of staining up to 500 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable