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1.00092

Millipore

FRASER Listeria Ammonium iron (III) Supplement

for Listeria spp., pkg of 10 vials, for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base)

同義詞:

FRASER Listeria Ammonium iron(III) Supplement, Listeria Selective Supplement

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About This Item

經驗公式(希爾表示法):
C6H8O7 · xFe · xH3N
CAS號碼:
MDL號碼:
分類程式碼代碼:
41106212
酶委員會索引編號:
214-686-6
NACRES:
NA.74

agency

ISO 11290
ISO

品質等級

描述

GranuCult™ Half FRASER (Demi FRASER) Broth (Base) (1.00025), GranuCult™ FRASER Broth (Base) (1.10398)

形狀

lyophilized powder

效力

>2000 mg/kg LD50, oral (Rat)

成份

Ammonium iron (III) citrate, 250 mg per vial

包裝

pkg of 10 vials

技術

microbiological culture: suitable

pH值

6-8 (20 °C, 100 g/L in H2O)

溶解度

1200 g/L

應用

food and beverages
microbiology
pathogen testing

儲存溫度

15-25°C

適合性

Listeria spp.

InChI

1S/C6H8O7.Fe.H3N/c7-3(8)1-6(13,5(11)12)2-4(9)10;;/h13H,1-2H2,(H,7,8)(H,9,10)(H,11,12);;1H3/q;+3;/p-3

InChI 密鑰

FRHBOQMZUOWXQL-UHFFFAOYSA-K

一般說明

For preparation of FRASER broth, two different supplements are needed: FRASER Listeria Selective Supplement and FRASER Listeria Ammonium Iron (III) Supplement. The ammonium iron (III) citrate promotes the growth of the Listeria spp., and together with esculin (a component of the FRASER broth base), permits the β-D-glucosidase detection in Listeria spp.

應用

FRASER Listeria Ammonium iron (III) Supplement is an additive for the preparation of Half FRASER (Demi FRASER) Broth (Base) or FRASER Broth (Base) for the enrichment of Listeria spp.

聯結

Replaces: 1000920017

分析報告

Growth promotion test according to ISO 11133
Mixed inoculum of Listeria, E.coli and E.faecalis
Additionaly monoculture of E.coli and E.faecalis
Pre-enrichment in Half Fraser Broth, afterwards enrichment in Fraser Broth
Confirmation on ALOA®-Medium resp. Tryptic soy agar
Inoculum on reference medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
10 - 100
- Listeria monocytogenes ATCC 13932 (WDCM 00021): 10 - 100
- Escherichia coli ATCC 25922 (WDCM 00013): ≥ 1000
- Escherichia coli ATCC 8739 (WDCM 00012): ≥ 1000
- Enterococcus faecalis ATCC 19433 (WDCM 00009): ≥ 1000
- Enterococcus faecalis ATCC 29212 (WDCM 00087): ≥ 1000
Blacking
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
+
- Listeria monocytogenes ATCC 13932 (WDCM 00021): +
- Escherichia coli ATCC 25922 (WDCM 00013): -
- Escherichia coli ATCC 8739 (WDCM 00012): -
- Enterococcus faecalis ATCC 19433 (WDCM 00009): -
- Enterococcus faecalis ATCC 29212 (WDCM 00087): -
Growth on ALOA medium
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
>10 CFU
- Listeria monocytogenes ATCC 13932 (WDCM 00021): >10 CFU
Colonies
- Listeria monocytogenes ATCC 19111 (WDCM 00020):
bluegreen with non transparent zone
- Listeria monocytogenes ATCC 13932 (WDCM 00021): bluegreen with non transparent zone
Growth on Tryptic soy agar
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): <100 CFU
- Enterococcus faecalis ATCC 19433 (WDCM 00009): <100 CFU
Incubation:
Half Fraser Broth: 24 hours at 30°C, aerobic
Fraser Broth: 48 hours at 37°C, aerobic
Confirmation on ALOA-Medium: 48 hours at 37°C, aerobic
Confirmation on Tryptic soy agar: 24 hours at 35°C, aerobic

法律資訊

ALOA is a registered trademark of Biolife Italiana S.r.l.

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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文章

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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