07-1594

Anti-Phospho-Dnmt1(Ser714) Antibody

from rabbit

• 同義詞: CXXC finger protein 9, CXXC-type zinc finger protein 9, DNA (cytosine-5-)-methyltransferase 1, DNA MTase HsaI, DNA methyltransferase 1, DNA methyltransferase HsaI
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human
• 技術: ELISA: suitable; dot blot: suitable; western blot: suitable
• NCBI登錄號: NP_001370
• UniProt登錄號: P26358
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer714)
• 基因資訊: human ... DNMT1(1786)

描述

一般說明

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes may be linked with development of cancer. Three families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of Dnmt1 causes global demethylation of genomic DNA and embryonic lethality. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Phosphorylation of Ser714 is induced by EGF, but the role of this modification is unknown.

特異性

Not tested in other species.

This antibody recognizes human Dnmt1 phosphorylated on Ser714.

免疫原

Epitope: Ser714

The immunogen was a KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser714 of human Dnmt1.

應用

Anti-Phospho-Dnmt1(Ser714) Antibody is a rabbit polyclonal antibody for detection of Phospho-Dnmt1(Ser714) also known as CXXC finger protein 9, DNA (cytosine-5-)-methyltransferase 1 & DNA MTase HsaI has been validated in WB, ELISA, DB.

ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).

Dot blotting:
This antibody has been shown by an outside laboratory to be specific for the phospho-Ser714 immunogen peptide, but not the unphosphorylated peptide, by dot blotting (1:500).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

品質

Western Blot Analysis:
1:250 dilution of this antibody detected Dnmt1 on 10 µg HeLa nuclear extract

標靶描述

~184 kDa

外觀

Purified

Format: Purified

Purified rabbit polyclonal in buffer containing 0.09% NaN3.

儲存和穩定性

Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
HeLa nuclear extract or EGF-treated whole HeLa cell extract.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable