06-1063

Anti-Frizzled-7 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: frizzled homolog 7, Frizzled, homolog of, 7, frizzled-7, frizzled homolog 7
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: rat, human, mouse
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_003498.1
• UniProt登錄號: O75084
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FZD7(8324)

描述

一般說明

Frizzled 7 is a multi-pass membrane protein belonging to the G-protein coupled receptor Fz/Smo family. It is a receptor for Wnt signaling, predominantly Wnt11, a crucial factor in the regulation of cellular migration and polarization during vertebrate gastrulation. Highest reported expression is in fetal kidney tissue and adult heart tissue. Lower levels have been observed in brain, placenta, adult heart, fetal lung, and squamous cell esophageal carcinomas.

特異性

Recognizes Frizzled-7

免疫原

Epitope: Unknown

KLH-conjugated linear peptide corresponding to human Frizzled-7.

應用

Research Category
Signaling

Research Sub Category
Developmental Signaling

This Anti-Frizzled-7 Antibody is validated for use in WB, IH, IC for the detection of Frizzled-7.

Western Blot Analysis: A previous lot detected Frizzled-7 on 10 µg of HEK293 cell lysate and rat brain microsomal fraction tissue lysate.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Frizzled-7 in human ductal in situ and GI tract tissue.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Frizzled-7 in A431 cells.

品質

Evaluated by Western Blot in mouse brain microsomal fraction tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Frizzled-7 on 10 µg of mouse brain microsomal fraction tissue lysate.

標靶描述

The calculated molecular weight is 64 kDa Observed at ~83 kDa, most likely due to multiple glycosolation sites.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Mouse brain microsomal fraction tissue lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable