05-157
Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9
clone 1B3B9, Upstate®, from mouse
同義詞:
Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK 2, Mitogen-activated protein kinase 2, extracellular signal-regulated kinase-2, mitogen-activated protein kinase 1, protein tyrosine kinase ERK2.
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About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
1B3B9, monoclonal
物種活性
avian, mouse, human, rat
製造商／商標名
Upstate®
技術
immunoprecipitation (IP): suitable
western blot: suitable
同型
IgG2a
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... MAPKAPK2(9261)
mouse ... Mapkapk2(17164)
rat ... Mapkapk2(289014)
一般說明
ERK2 is activated by MEK2, which phosphorylates neighboring threonine 183 and tyrosine 185 residues. ERK2 is a serine/threonine kinase that phosphorylates MAP2 and myelin basic protein. This kinase is an important proximal component of the MAP kinase pathway involved in transeitting the signals from growth factors, neurotransmitters and hormones at the cell surface to the transcriptional events in the nucleus.
特異性
Other species cross reactivity is unknown.
This antibody recognizes MAP kinase encoded by the mapk gene, Mr 42 kDa.
免疫原
HPLC purified murine recombinant MAP Kinase. Clone 1B3B9
應用
Anti-MAP Kinase 2/Erk2 Antibody, clone 1B3B9 is an antibody against MAP Kinase 2/Erk2 for use in IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated MAP Kinase 2/Erk2 from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody, as this antibody only binds to denatured MAP Kinase.
4 μg of a previous lot immunoprecipitated MAP Kinase 2/Erk2 from a RIPA lysate from mouse 3T3 cells. Boil the cell lysate and let cool before adding the antibody, as this antibody only binds to denatured MAP Kinase.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
品質
Routinely evaluated in RIPA lysates from human A431 carcinoma cells, mouse 3T3 fibroblasts, or rat L6 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected MAP Kinase 2/Erk2 in RIPA lysates from human A431 carcinoma cells. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected MAP Kinase 2/Erk2 in RIPA lysates from human A431 carcinoma cells. A previous lot detected MAP Kinase in mouse 3T3 fibroblasts and rat L6 cells.
標靶描述
42 kDa
聯結
Replaces: 04-349
外觀
Protein G Chromatography
Format: Purified
Purified mouse monoclonal IgG2a in buffer containing PBS with 0.05% sodium azide. Store frozen at -20°C, avoid freeze thaw cycles.
儲存和穩定性
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Mitogen-activated protein kinase modulates ethanol inhibition of cell adhesion mediated by the L1 neural cell adhesion molecule.
Dou, X; Wilkemeyer, MF; Menkari, CE; Parnell, SE; Sulik, KK; Charness, ME
Proceedings of the National Academy of Sciences of the USA null
Allelic variation of the ACCase gene and response to ACCase-inhibiting herbicides in pinoxaden-resistant Lolium spp.
Laura Scarabel,Silvia Panozzo,Serena Varotto,Maurizio Sattin
Pest Management Science null
Reversion of Ras- and phosphatidylcholine-hydrolyzing phospholipase C-mediated transformation of NIH 3T3 cells by a dominant interfering mutant of protein kinase C lambda is accompanied by the loss of constitutive nuclear mitogen-activated protein kinase/extracellular signal-regulated kinase activity.
Bjorkoy, G, et al.
The Journal of Biological Chemistry, 272, 11557-11565 (1997)
Cytosolic phospholipase A2 is phosphorylated in collagen- and thrombin-stimulated human platelets independent of protein kinase C and mitogen-activated protein kinase.
Borsch-Haubold, A G, et al.
The Journal of Biological Chemistry, 270, 25885-25892 (1995)
Y Ihara et al.
The Journal of biological chemistry, 272(15), 9629-9634 (1997-04-11)
beta-1,4-N-Acetylglucosaminyltransferase III (GnT-III: EC 2.4.1.144) is a pivotal glycosyltransferase which participates in branch formation by catalysis of the synthesis of a bisecting GlcNAc structure in N-glycans. These structures are thought to be one of the unique features of the N-glycans
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