05-166

Anti-IGF-II Antibody, clone S1F2

clone S1F2, Upstate^®, from mouse

• 同義詞: Anti-C11orf43, Anti-GRDF, Anti-IGF-II, Anti-PP9974, Anti-SRS3
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: S1F2, monoclonal
• 物種活性: human, rat
• 製造商／商標名: Upstate^®
• 技術: ELISA: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; neutralization: suitable; western blot: suitable
• 同型: IgG1
• NCBI登錄號: NM_000612
• UniProt登錄號: P01344
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... IGF2(3481)

描述

一般說明

Human Insulin-like Growth Factor II (IGFII) contains 67 amino acids and shares similar structural features with IGFI, including a 62% sequence homology. In human plasma, IGFI and IGFII are associated with IGF-binding proteins that transport the polypeptides and partially regulate their actions in vivo. In addition to the insulin receptor, IGFII binds to two forms of IGF receptors, both of which are widely distributed in different tissues and cultured cells. IGFII is mitogenic for a variety of cultured cells, including mouse 3T3 cells,12 normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells.

特異性

Less than 10% cross-reactivity to human IGF-I (Tanaka, H, 1989; Suzuki, T, 1989).

This antibody detects IGF-II.

免疫原

Partially purified rat IGF-II. Clone S1F2

應用

Detect IGF-II using this Anti-IGF-II Antibody, clone S1F2 validated for use in ELISA, IP, IC, NEUT & WB.

Neutralization: 10 μg/mL of a previous lot inhibited the stimulatory effects of IGF-II (10 ng/mL) on DNA synthesis in chicken embryo fibroblasts. Cell viability was assessed using an ATP endpoint assay (ATPLiteÔ-M, Packard Instruments). Previous lots of antibody were tested to inhibit IGF-II-dependent incorporation of [3H]-thymidine

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

品質

Evaluated by Western Blotting on HeLa acid extracted nuclear preps (positive) and recombinant Histone H3 (negative).

Western Blotting Analysis:
1:5,000 – 1:10,000 dilution of this antibody was used to detect trimethylated-Histone H3 (Lys4) in HeLa nuclear acid extracts.

標靶描述

~7 kDa

外觀

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing PBS, pH 7.4., purified by ammonium sulfate precipitation and DEAE-cellulose chromatography. Frozen solution.

儲存和穩定性

Stable for 1 year at -20ºC from date of receipt.

分析報告

Control
Human head and neck tumours, angioma tissues

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable