04-772
Anti-Src Antibody, CT, clone NL19 | 04-772
clone NL19, Upstate®, from rabbit
同義詞:
Proto-oncogene tyrosine-protein kinase SRC, Protooncogene SRC, Rous sarcoma tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
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About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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一般說明
SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160 kDa and has a basic helix-loop-helix (bHLH)-PAS domain.
特異性
Predicted to cross react with frog and chicken. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.
Src-tyrosine kinase family
免疫原
KLH conjugated synthetic peptide (CKVADFGLARLIEDNEYTARQGA) of Src (avian sarcoma virus), residues 400-422. The immunogen sequence shares 22/23 amino acids with Yes and Fyn, and 21/23 amino acids with Lyn and Hck.
應用
Western Blot Analysis: A 1:2000 to 1:8000 dilution of this lot detected Src in RIPA whole cell lysates from human A431 cells. Immunoprecipitation: 5-20 μL of a previous lot immunoprecipitated Src from 500 μg of A431 RIPA lysate.
品質
routinely evaluated by immunoblot on RIPA whole cell lysates from human A431 and Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells
標靶描述
~53-62kDa
聯結
Replaces: 05-772
外觀
Format: Purified
分析報告
Control
RIPA whole cell lysates from human A431, Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells Included Positive Antigen Control: Catalog # 12-301, Non-Stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
RIPA whole cell lysates from human A431, Jurkat cells, mouse 3T3/A31 cells or rat PC-12 cells Included Positive Antigen Control: Catalog # 12-301, Non-Stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Machika Kawamura et al.
Life science alliance, 4(8) (2021-06-26)
The pericentromeric heterochromatin of one-cell embryos forms a unique, ring-like structure around the nucleolar precursor body, which is absent in somatic cells. Here, we found that the histone H3 variants H3.1 and/or H3.2 (H3.1/H3.2) were localized asymmetrically between the male
Pazit Y Cohen et al.
American journal of respiratory cell and molecular biology, 40(2), 231-238 (2008-08-05)
We have previously demonstrated that myofibroblasts from lungs with bleomycin-induced fibrosis overexpress FasL molecules. Two subpopulations of fibroblasts, distinguished by their expression of Thy1 molecules, have been shown in the lungs of both mice and humans. Thy1-mediated FasL induction has
Amélie Juin et al.
The Journal of cell biology, 207(4), 517-533 (2014-11-26)
Accumulation of type I collagen fibrils in tumors is associated with an increased risk of metastasis. Invadosomes are F-actin structures able to degrade the extracellular matrix. We previously found that collagen I fibrils induced the formation of peculiar linear invadosomes
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