806536

APN-Maleimide

• 同義詞: 3-(4-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)phenyl)propiolonitrile, MAPN
• 經驗公式（希爾表示法）: C₁₃H₆N₂O₂
• 分子量：: 222.20
• 分類程式碼代碼: 12352111
• PubChem物質ID: 329769017
• NACRES: NA.22

屬性

• 形狀: powder
• 品質等級: 100
• 儲存溫度: 2-8°C
• SMILES 字串: N#CC#CC1=CC=C(N2C(C=CC2=O)=O)C=C1
• InChI: 1S/C13H6N2O2/c14-9-1-2-10-3-5-11(6-4-10)15-12(16)7-8-13(15)17/h3-8H
• InChI 密鑰: CHKKXKRQICWZFF-UHFFFAOYSA-N

描述

應用

APN-Maleimide is a bifunctional crosslinker for thiol-to-thiol coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. Due to kinetic resolution the first thiol reacts exclusively with the maleimide residue producing the protein-APN conjugate. This conjugate can be readily coupled with thiol-containing molecules.

準備報告

Standard protein labeling procedure (cysteine labeling).

• Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 10 minutes.
• If necessary, purify the protein-APN conjugate using size exclusion chromatography or ultrafiltration.
• The conjugate can be readily coupled with thiol-containing substrates by incubating the components in aqueous buffer (pH 6.5-9.0) at ambient temperature for 2 hours.

*Note: avoid thiol-containing buffers.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H302,H315,H319,H335
• 防範說明: P261 - P264 - P270 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• 危險分類: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• 標靶器官: Respiratory system
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable