568323-U
Discovery® BIO Wide Pore C8 HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mm
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About This Item
material
stainless steel column
Quality Level
Agency
suitable for USP L7
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
5% Carbon loading
parameter
0-70 °C temperature
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
surface area
100 m2/g
surface coverage
4 μmol/m2
matrix
silica particle platform
fully porous particle
matrix active group
C8 (octyl) phase
particle size
5 μm
pore size
300 Å
operating pH
2-8
separation technique
reversed phase
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General description
Discovery BIO Wide Pore C8 exhibits hydrophobicity intermediate between the Discovery BIO Wide Pore C5 and the Discovery BIO Wide Pore C18. The difference in hydrophobicity gives it unique selectivity relative to these other phases. It is ideal for peptide mapping because it provides complementary information compared to a C18 separation. Because of its intermediate hydrophobicity, we also recommend it for method development or scouting work. As with all Discovery BIO Wide Pore phases, the C8 phase gives efficient, symmetrical peaks, exceptional stability, long column lifetime, and LC-MS compatibility.
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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