56103AST

apHera^™ C18 (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

• Synonym(s): Polymeric C18 Reversed-Phase Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: apHera^™ C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
• material: stainless steel column
• Agency: suitable for USP L67
• product line: apHera^™
• packaging: pkg of 1 ea
• manufacturer/tradename: apHera^™
• parameter: 147 bar pressure (2133 psi); 4-60 °C temperature
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 4.6 mm
• matrix: polymeric
• matrix active group: C18 (octadecyl) phase
• particle size: 5 μm
• pore size: 300 Å
• operating pH: 1-14
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

apHera^™ reversed columns were devoloped specifically to provide the superior advantages of both silica and polystyrene columns, without the disadvantages of either. This was accomplished using a vinyl alcohol copolymer base that keeps the surface wetted even with high carbon loads. The porous structure has an average pore diameter large enough to produce ideal results for small analytes, peptides and small proteins. These columns equal silica based columns in separation efficiency with organic solvents but provide efficiency with buffered alkaline solutions not possible on silica. One of the most significant features is logicial elution order of alkylated bases where retention increases proportionately with increasing chain length.

Features of apHera^™ Reversed Phase Columns

• Stable vinyl copolymer base
• Stable pH range 2-12
• 300 angstrom pore size
• Stable in all organic solvents
• Expanded applications: peptides, proteins
• Efficient separation of any basic substance
• Amenability to washing and alkaline solutions

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Legal Information

apHera is a trademark of Sigma-Aldrich Co. LLC