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Key Documents

T1637

Sigma-Aldrich

N-(p-Tosyl)-Gly-Pro-Arg p-nitroanilide acetate salt

thrombin substrate

Synonym(s):

N-Tosylglycyl-L-prolyl-L-arginine 4-nitroanilide acetate salt

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About This Item

Linear Formula:
C26H34N8O7S · CH3COOH
CAS Number:
Molecular Weight:
662.71
Beilstein:
8891305
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:

Assay

≥98% (TLC)

form

powder

solubility

ethanol: 20 mg/mL

storage temp.

−20°C

SMILES string

CC(O)=O.Cc1ccc(cc1)S(=O)(=O)NCC(=O)N2CCC[C@H]2C(=O)N[C@@H](CCCNC(N)=N)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C26H34N8O7S.C2H4O2/c1-17-6-12-20(13-7-17)42(40,41)30-16-23(35)33-15-3-5-22(33)25(37)32-21(4-2-14-29-26(27)28)24(36)31-18-8-10-19(11-9-18)34(38)39;1-2(3)4/h6-13,21-22,30H,2-5,14-16H2,1H3,(H,31,36)(H,32,37)(H4,27,28,29);1H3,(H,3,4)/t21-,22-;/m0./s1

InChI key

NGXDRWVTRQRSED-VROPFNGYSA-N

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Substrates

A chromogenic substrate for thrombin.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Rui Nian et al.
Journal of chromatography. A, 1214(1-2), 47-58 (2008-11-14)
Gloshedobin, a recently isolated thrombin-like enzyme from the snake venom of Gloydius shedaoensis, is expressed mainly in the form of inclusion bodies (IBs) in Escherichia coli due to its cysteine-rich nature. Following extraction and solubilization of the IBs, one-step immobilized
Antithrombin (heparin cofactor) assay with "new" chromogenic substrates (S-2238 and Chromozym TH).
U Abildgaard et al.
Thrombosis research, 11(4), 549-553 (1977-10-01)
Qi-Juan Gu et al.
Journal of insect physiology, 116, 41-48 (2019-04-27)
To establish successful infections, endoparasitoid wasps must develop strategies to evade immune responses of the host. Here, we identified and characterized a teratocytes-expressed gene encoding a trypsin inhibitor-like protein containing a cysteine-rich domain from Cotesia vestalis, CvT-TIL. CvT-TIL had a
Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates.
R Lottenberg et al.
Methods in enzymology, 80 Pt C, 341-361 (1981-01-01)

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