SRP4324
Apo-SAA human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Synonym(s):
Amyloid fibril protein AA, Amyloid protein A, SAA, SAA1, SAA2, Serum amyloid A protein
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About This Item
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biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
mol wt
~11.5 kDa
packaging
pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
protein expression: suitable
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... SAA1(6288)
General description
Human apo-SAA is a 104 amino acid polypeptide that circulates primarily in association with high-density lipoproteins (HDL). The level of apo-SAA, normally 1-5 μg/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL apo-lipoprotein. The human SAA gene codes for a 122 amino acid polypeptide, which contains an 18 amino acid N-terminal signal sequence.
Application
Apo-serum amyloid A has been used to study its effect on the expression of Visfatin in macrophages.
Biochem/physiol Actions
Serum amyloid A (SAA) is found normally at concentrations of 0.1μM in blood. However, its concentration increases up to 1000-fold under stress. It has been associated with several chronic inflammatory diseases, such as atherosclerosis. It is found to participate in the inflammatory response by facilitating chemotaxis, migration, and adhesion of inflammatory cells, particularly monocytes/macrophages. SAA is also found to induce the synthesis and secretion of multiple kinds of inflammatory cytokines, such as TNF-α (tumor necrosis factor-α), IL-1β (interleukin-1β), MCP-1 (monocyte chemoattractant protein-1), and Lp-PLA2 (lipoprotein-associated phospholipase A2).
Physical form
Sterile filtered and Lyophilized without additives.
Reconstitution
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in 0.1% acetic acid to a concentration of 1 μg/ul. This solution can then be diluted into other aqueous buffers.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Serum Amyloid a Promotes Visfatin Expression in Macrophages.
Wang S
BioMed Research International (2016)
Kiyoshi Migita et al.
PloS one, 9(5), e96703-e96703 (2014-05-23)
Serum amyloid A (SAA) is an acute phase reactant with significant immunological activities, including effects on cytokine synthesis and neutrophil chemotaxis. Neutrophils can also release cytokines with proinflammatory properties. IL-1β is a key proinflammatory cytokine, the secretion of which is
Shixun Wang et al.
BioMed research international, 2016, 4819327-4819327 (2016-03-24)
Visfatin has been reported to exert an important role in the development of atherosclerosis. However, the mechanism that regulated the expression of Visfatin has not been elucidated yet. This study aimed to investigate the effect of SAA on the regulation
Qian Yan et al.
Cellular signalling, 26(9), 1783-1791 (2014-04-08)
Serum amyloid A (SAA), a major acute-phase protein, has potent cytokine-like activities in isolated phagocytes and synovial fibroblasts. SAA-induced proinflammatory cytokine gene expression requires transcription factors such as NF-κB; however, the associated epigenetic regulatory mechanism remains unclear. Here we report
Lei Sun et al.
European journal of immunology, 44(7), 2153-2164 (2014-04-30)
Interleukin-33 (IL-33), an IL-1 family cytokine and nuclear alarmin, is constitutively expressed in epithelial barrier tissues and human blood vessels. However, little is known about the induced expression of IL-33 in monocytes and macrophages, which are major cytokine-producing cells of
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