EZGLP1T

Multi Species GLP-1 ELISA Kit

measures total GLP-1 levels in 20 µl - 50 µl serum, plasma or cell culture supernatent

• Synonym(s): Glucagon-like peptide-1
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.32

Properties

• product name: Multi Species GLP-1 Total ELISA, EZGLP1T-36K, This Multi Species GLP-1 Total ELISA, EZGLP1T-36K, is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism research.
• Quality Level: 200
• species reactivity: human, mouse, rat
• packaging: kit of 1 × 96 wells
• parameter: 20—50 μL sample volume (4hr assay)
• assay range: linearity: 90-132%; recovery range: 90-110%; sensitivity: 1.5 pM; (MinDC); standard curve range: 4.1-1000 pM; inter-assay cv: <12%; intra-assay cv: <5%
• technique(s): ELISA: suitable
• input: sample type cell culture supernatant; sample type plasma (K2 EDTA); sample type serum
• NCBI accession no.: NM_002054.2
• UniProt accession no.: P01275
• application(s): research use
• detection method: colorimetric (450nm/590nm)
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: human ... GCG(2641)

Description

General description

Glucagon-like peptide-1 (GLP-1) is a 30 amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after cleavage by DPPIV. GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity. This kit measures both the active (7-36) and inactive (9-36) forms of GLP-1.

Specificity

Cross Reactivty
GLP-1 is conserved in almost all mammals.

The antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.

Application

Research Category
Metabolism

This is a 3.5-hour assay at room temperature requiring 50 uL of serum, plasma or culture samples per well.

Used to detect/quantify: Glucagon Like Peptide-1

Disclaimer

For research use only. Not for use in diagnostic procedures.

Safety Information

• Pictograms: GHS05,GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H290 - H302 + H332 - H311 - H317 - H411
• Precautionary Statements: P234 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3