F4042

Monoclonal ANTI-FLAG^® M5 antibody produced in mouse

clone M5, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-ddddk, Anti-dykddddk
• MDL number: MFCD00282246
• UNSPSC Code: 12352203
• NACRES: NA.32

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: M5, monoclonal
• form: buffered aqueous solution
• purified by: using Protein A
• species reactivity: all
• concentration: 2-5 mg/mL
• technique(s): western blot (chemiluminescent): 10 μg/mL
• isotype: IgG1
• immunogen sequence: DYKDDDDK
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Monoclonal ANTI-FLAG^® M5 is a mouse antibody that binds to N-terminal Met-FLAG fusion proteins. It is useful for detection of N-terminal Met-FLAG fusion proteins expressed in mammalian and Drosophilae cells.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG^® M5 antibody produced in mouse has been used in western blotting and immunofluorescence.

Binds the FLAG peptide only when it is located at the amino terminus preceded by a methionine. Binding is not Ca²⁺-dependent. Useful for detecting cytoplasmically expressed Met-FLAG fusion proteins in mammalian crude cell extracts, but not recommended for fusion proteins expressed in E. coli.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v)

Preparation Note

Dilute the antibody to 10 mg/mL in Tris buffered saline (TBS): 0.05 M Tris, 0.15 M NaCl, pH 7.4.

Other Notes

Antibody is not calcium dependent.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable