475904-M

MOWIOL^® 4-88 Reagent

• Synonym(s): Poly(vinyl alcohol)
• Linear Formula: [-CH₂CHOH-]_n
• CAS Number: 9002-89-5
• MDL number: MFCD00081922
• UNSPSC Code: 12352200

Properties

• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: yellow
• storage temp.: 10-30°C
• InChI: 1S/C2H4O/c1-2-3/h2-3H,1H2
• InChI key: IMROMDMJAWUWLK-UHFFFAOYSA-N

Description

General description

Used for mounting cells on slides for detection of cell-surface antigens by immunofluorescence. Useful as an anti-fading agent.

Warning

Toxicity: Standard Handling (A)

Preparation Note

Add 2.4 g of MOWIOL^® 4-88 to 6 g of glycerol and stir to mix. Add 6 ml of water and stir for several hours at room temperature. Add 12 ml of 0.2 M Tris (pH 8.5) and heat to 50°C for 10 min. with occasional mixing. After the MOWIOL 4-88 dissolves, clarify the solution by centrifugation at 5000 x g for 15 min.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 weeks at 4°C.

Other Notes

Longin, A., et al. 1993. J. Histochem. Cytochem. 41, 1833.
Beug, H., et al. 1979. Cell18, 375.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Mowiol is a registered trademark of Kuraray Europe GmbH

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1