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G005396

HPLC Analysis of Chinese Ginseng Extract on Ascentis® Express C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A] water; [B] acetonitrile
gradient: held at 25% B for 1.5 min; 25 to 85% B in 12 min; held at 85% for 1 min
flow rate: 1 mL/min
pressure: 3481 psi (240 bar)
column temp.: 60 °C
detector: UV, 205 nm
injection: 25 μL
sample: Extracted from dried, ground plant material; 10 mg/mL in water:acetonitrile (85:15); sonicate 10 min at ambient

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of Chinese ginseng root extract.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Gensenoside Re
    Gensenoside Rg1
    Gensenoside Rf
    Gensenoside Rb1
    Gensenoside Rc
    Gensenoside Rb2
    Gensenoside Rd

analytical column

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Description
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