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53838-U

Supelco

Ascentis® Express 90Å C8 (2.7 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core C8 HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express C8, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

100

Agency

suitable for USP L7

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

C8 (octyl) phase

particle size

2.7 μm

pore size

90 Å

operating pH range

2-9

application(s)

food and beverages

separation technique

reversed phase

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General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Application


  • Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review explores the evolution and performance of superficially porous silica columns in high-performance liquid chromatography (HPLC), including those similar to the Ascentis Express C8, 2.7 μm. The focus is on the technology′s impact on enhancing analytical speed and efficiency (Ali et al., 2012).

  • Development and validation of an HPLC-MS/MS method to determine clopidogrel in human plasma. Use of incurred samples to test back-conversion.: This study details the development of an HPLC-MS/MS analytical method using columns such as the Ascentis Express C8, 2.7 μm, aimed at quantifying clopidogrel in plasma, demonstrating the column′s utility in clinical pharmacokinetic assessments (Silvestro et al., 2010).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
UPLC is a registered trademark of Waters

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
USZB005150
USZB005146
USZB005145
USZB005143
USZB005142

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Ugandar Reddy Inugala et al.
Journal of chromatographic science, 51(5), 453-459 (2012-10-13)
This paper describes the development of a rapid, novel, stability-indicating gradient reversed-phase high-performance liquid chromatographic method and associated system suitability parameters for the analysis of naproxcinod in the presence of its related substances and degradents using a quality-by-design approach. All
Sreenivasa Rao Chitturi et al.
Journal of pharmaceutical and biomedical analysis, 55(1), 31-47 (2011-02-15)
This paper proposes a simple and selective RP-HPLC method for the determination of process impurities and degradation products (degradants) of atazanavir sulfate (ATV) drug substance. Chromatographic separation was achieved on Ascentis(®) Express C8, (150mm×4.6mm, 2.7μm) column thermostated at 30°C under
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 81-82, 126-132 (2013-05-07)
A closed-vessel microwave-assisted extraction (MAE) technique was optimized for the first time for the extraction of polyphenols from raw propolis. The results obtained by means of response surface experimental design methodology showed that the best global response was reached when
Monika A Olszewska
Journal of separation science, 35(17), 2174-2183 (2012-07-19)
An HPLC method of high resolution has been developed and validated for the simultaneous determination of ten prominent flavonoid aglycones in plant materials using a fused-core C18-silica column (Ascentis® Express, 4.6 mm × 150 mm, 2.7 μm). The separation was
Imran Ali et al.
Biomedical chromatography : BMC, 26(8), 1001-1008 (2012-01-13)
Superficially porous silica particles columns (SPSPCs) are manufactured by different companies. The most common have the brand names Halo, Ascentis Express and Kinetex. These columns provide super fast, sharp peaks and moderate sample loading and back pressure. These are available
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Articles

Serum Lipidomics Profiling using an Ascentis Express Fused-Core C18 Column

Sigma-Aldrich presents an article on serum lipidomics profiling using an Ascentis Express Fused-Core C18 column.

Ascentis® Express C8 U/HPLC Columns

When a C18 doesn′t give the desired separation, or your sample contains compounds that are known to be difficult to retain or resolve on a C18, consider changing to an Ascentis® Express C8 column.

Protocols

US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18

-methylcarbamate 10 μg/mL; Diuron; Propham; Siduron; Methiocarb, analytical standard; Linuron 10 μg/mL; Swep 10 μg/mL; Chlorpropham 10 μg/mL; Barban; Neburon

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