O2260

Osteopontin from mouse

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• Synonym(s): Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
• MDL number: MFCD03100698
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: mouse
• Quality Level: 100
• recombinant: expressed in NSO cells
• sterility: sterile
• Assay: >95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 30 kDa by SDS-PAGE (reducing); 65 kDa by SDS-PAGE (reducing) (doublet)
• packaging: pkg of 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P10923
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: mouse ... Spp1(20750)

Description

Application

Osteopontin from mouse has been used:

• to study its role in a mouse model of preterm brain injury
• as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques
• to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels

Biochem/physiol Actions

Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable