SAB4200018

Anti-Osteopontin antibody, Mouse monoclonal

clone OPN46, purified from hybridoma cell culture

• Synonym(s): Anti-BNSP, Anti-Bone Sialoprotein 1, Anti-Early T Lymphocyte Activation Protein 1 (ETA1), Anti-OPN, Anti-SPP1, Anti-Secreted phosphoprotein 1, Anti-Urinary stone protein, Anti-Uropontin
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: OPN46, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: human, mouse, monkey, rat, canine
• concentration: ~1.0 mg/mL
• technique(s): western blot: 5-10 μg/mL using HepG2 cells extract
• isotype: IgG1
• UniProt accession no.: P10451
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SPP1(6696)

Description

General description

Monoclonal Anti-Osteopontin (mouse IgG1 isotype) is derived from the hybridoma OPN46 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human osteopontin. Osteopontin (OPN) is a secreted, integrin-binding glycophosphoprotein which serves as a cytokine and as an extracellular matrix molecule. It is produced by multiple tissues in the body and is most abundant in bone.

Application

Monoclonal Anti-Osteopontin antibody produced in mouse may be used in immunoblotting.

Biochem/physiol Actions

Osteopontin (OPN) synthesized in cancer cells is essentail for growth, progression and metastasis of cancer. In tuomr cells OPN is highly expressed The structural features of OPN differ between normal and tumor cells Three osteopontin splice variant transcripts have been found to be expressed in invasive, but not in non-invasive breast tumor cell lines. Osteopontin-b lacks exon 5 and osteopontin-c lacks exon 4. The shortest splice variant, osteopontin-c may support breast tumor progression by conveying anchorage independence and inducing oxidoreductases expression. It was also suggested as a diagnostic and prognostic breast cancer marker.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable