AB1879

Anti-Caspase 8 Antibody

Chemicon^®, from rabbit

• Synonym(s): MACH1, FLICE
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: monkey, bovine, rat, human, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• NCBI accession no.: NM_001080124.1; NM_001080125.1; NM_001228.4; NM_033355.3; NM_033356.3; NM_033358.3
• UniProt accession no.: Q14790
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CASP8(841) ; mouse ... Casp8(12370)

Description

General description

Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive proenzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 kDa fragment and a 12 kDa which is further processed to 10 kDa, and then the p43 is then cleaved to yield p26 and the release of the active site containing p18.

Specificity

AB1879 detects 55 and 43 kDa proteins, corresponding to the proform and large subunit of caspase-8. The antibody also detects human recombinant caspase-8 protein on SDS-PAGE immunoblots.

Immunogen

recombinant, full length, purified human caspase 8

Application

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Caspases

Enzymes & Biochemistry

This Anti-Caspase 8 Antibody is validated for use in WB for the detection of Caspase 8.

Western blot: 0.5 - 4 μg/mL

Optimal working dilutions must be determined by end user.

Target description

55 and 43 kDa

Linkage

Replaces: 04-573; 04-574

Physical form

ImmunoAffinity Purified

Liquid in PBS, pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thiomerosal. Affinity Purified immunoglobulin.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Skin/Tumor, Embryo

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2