Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

S1569

Sigma-Aldrich

Stemline® Mesenchymal Stem Cell Expansion Medium

Without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

MSC Medium

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352200
NACRES:
NA.81

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

Developed to promote the optimal expansion of human mesenchymal stem cells (MSC) from bone marrow, Stemline Mesenchymal Stem Cell Expansion Medium demonstrates greater total nucleated cell (TNC) fold increases than other commercially available formulations. Additionally, functional trials clearly demonstrated Stemline′s capacity to promote differentiation into adipocytes, chondrocytes, and osteocytes.

Stemline Mesenchymal Stem Cell Expansion Medium requires supplementation with antibiotics, cytokines, L-glutamine and fetal bovine serum, as appropriate to individual research protocols. Known to be extremely sensitive during initial isolation and growth ex vivo, MSC proliferation depends highly on the composition of fetal bovine serum (FBS) used to supplement their medium. Pre-screening with FBS is recommended, as the specific FBS components that effect MSC growth have not been fully identified.

Produced in a GMP state-of-the-art facility, Stemline Mesenchymal Stem Cell Expansion Medium is clearly an excellent choice for your MSC applications.

Reconstitution

Medium must be supplemented with antibiotics, cytokines, 4 mM L-glutamine, and fetal bovine serum according to individual user protocols.

Legal Information

Stemline is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

J Rodrigo Mora et al.
Science (New York, N.Y.), 314(5802), 1157-1160 (2006-11-18)
Normal intestinal mucosa contains abundant immunoglobulin A (IgA)-secreting cells, which are generated from B cells in gut-associated lymphoid tissues (GALT). We show that dendritic cells (DC) from GALT induce T cell-independent expression of IgA and gut-homing receptors on B cells.
I McNiece et al.
Cytotherapy, 6(4), 311-317 (2005-09-09)
Cord blood (CB) cells are being used increasingly as a source of hematopoietic cells to support high dose chemotherapy. However, CB units contain low numbers of cells, including CD34+ cells, and thus their use is associated with significant delays in

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Mesenchymal Stem Cell FAQs

Protocols

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service