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MAK014

Sigma-Aldrich

Glucose-6-Phosphate Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

hematological disorder

storage temp.

−20°C

General description

Glucose 6-phosphate (G6P) is a key metabolic intermediate that enters either metabolic pathways or storage. G6P is generated when glucose is phosphorylated by hexokinase or glucokinase or by the conversion of glucose-1-phosphate by phosphoglucomutase during glycogenolysis. G6P lies at the beginning of both glycolysis and the pentose phosphate pathways. It also can be stored as glycogen when blood glucose levels are high.

Application

Glucose-6-phosphate assay kit has been used in:

  • glucose 6-phosphate (G6P) assay
  • quantification of glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P)
  • metabolite analysis

Suitability

Suitable for quantifying Glucose-6-Phosphate in a variety of samples.

Principle

G6P is utilized by Glucose-6-phosphate dehydrogenase to generate reducing equivalents in the form of NADPH. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. G6P is determined by an enzyme assay, which results in a colorimetric (450 nm) product, proportional to the G6P present. Typical sensitivities of detection are between 1-30 nmoles.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H314,H334

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Renal Disease in Type I Glycogen Storage Disease
Richard P L, et al.
Genetic Diseases of the Kidney (2009)
Reciprocal Control of Anaplerotic Phosphoenolpyruvate Carboxylase by in Vivo Monoubiquitination and Phosphorylation in Developing Proteoid Roots of Phosphate-Deficient Harsh Hakea
Shane M W, et al.
Plant Physiology, 112-112 (2013)
MiR-182 promotes glucose metabolism by upregulating hypoxia-inducible factor 1a in NSCLC cells
Mingliang W, et al.
Biochemical and Biophysical Research Communications, 504(2), 400-405 (2018)
Saixiang Feng et al.
Veterinary research, 51(1), 97-97 (2020-08-02)
Lipooligosaccharides (LOSs) are virulence determinants of Glaesserella parasuis, a pathogen of the respiratory tract of pigs. We previously reported that disruption of the galU or galE gene in G. parasuis results in increased sensitivity to porcine serum, indicating that the
Forkhead Box O6 (FoxO6) Depletion Attenuates Hepatic Gluconeogenesis and Protects against Fat-induced Glucose Disorder in Mice.
Virtu C N, et al.
The Journal of Biological Chemistry, 290, 15581-15594 (2015)
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Articles

Aerobic Glycolysis and the Warburg Effect

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

Protocols

Enzymatic Method for Determining Glucose-6-Phosphate (Glucose-6-Phosphate Assay)

Enzymatic Method for Determining Glucose-6-Phosphate (Glucose-6-Phosphate Assay)

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