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C1099

Sigma-Aldrich

Caspase 8 human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine tagged), buffered aqueous solution, ≥1,000 units/mg protein

Synonym(s):

FLICE, MASH, Mch5

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About This Item

MDL number:
MFCD01865996
UNSPSC Code:
12352204
NACRES:
NA.32

recombinant

expressed in E. coli (C-terminal histidine tagged)

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

buffered aqueous solution

specific activity

≥1,000 units/mg protein

mol wt

~30 kDa

UniProt accession no.

Q14790

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CASP8(841)

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General description

Human recombinant C−terminal histidine tagged caspase 8 is expressed as the truncated pro-caspase 8 (amino acids 213−496). It is a fully active protein consisting of 18 kDa and 12.4 kDa subunits; the 12.4 kDa subunit contains the histidine tag.

Biochem/physiol Actions

Caspase 8 is at the top of the hierarchy of the caspase cascade and is considered an initiator caspase. It exists in the cell as an inactive 55 kDa proenzyme. It is converted to the active form, consisting of 18 kDa and 11 kDa subunits, upon its recruitment to the cytoplasmic domain of activated death receptors such as CD95 or TNFR via the adapter protein FADD. The activation of the proenzyme is triggered by the protein′s aggregation, which leads to auto- or transprocessing. Caspase 8 activates downstream caspases (caspases 3, 6, and 7) that cleave key cellular substrates and lead to apoptotic death of the cells.

Unit Definition

One unit will cleave 1.0 nmol of Ac-Ile-Glu-Thr-Asp-pNA per min at pH 7.4 at 25 °C.

Physical form

Solution in 10% sucrose containing 20 mM Tris-HCl, 150 mM imidazole-HCl, pH 8.0, 20 mM 2-mercaptoethanol, 500 mM NaCl, 2.5 mM EDTA, and 0.1% CHAPS.

Pictograms

Exclamation markHealth hazard
GHS07,GHS08

Signal Word

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A Prakasam et al.
Cell death & disease, 5, e1358-e1358 (2014-08-01)
BH3 interacting-domain death agonist (Bid) is a BH3-only pro-apoptotic member of the Bcl-2 family of proteins. Its function in apoptosis is associated with the proteolytic cleavage to the truncated form tBid, mainly by caspase-8. tBid translocates to mitochondria and assists

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