3HBDB-RO
Roche
3-Hydroxybutyrate Dehydrogenase (3-HBDH)
suspension, ~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.), grade II
Synonym(s):
3-Hydroxybutyrate Dehydrogenase (3-HBDH), 3-HBDH
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![Poly[(R)-3-hydroxybutyric acid] natural origin](/deepweb/assets/sigmaaldrich/product/structures/129/476/7d1c924b-f644-4889-a2d6-d7a923ce382c/640/7d1c924b-f644-4889-a2d6-d7a923ce382c.png)
Quality Level
form
suspension
specific activity
~3 units/mg protein (At 25 °C with 3-hydroxybutyrate as the substrate; standardized with BSA.)
packaging
vial of 2 mL (10127833001)
vial of 5 mL (10127841001)
manufacturer/tradename
Roche
concentration
5 mg/mL
impurities
<0.1% LDH
<5% MDH
optimum pH
6.2-6.9(for reduction)
7-9(for oxidation)
shipped in
wet ice
storage temp.
2-8°C
General description
3-Hydroxybutyrate dehydrogenase (3-HBDH) enzyme, obtained from Rhodobacter sphaeroides, is generally used for the quantification of ketone bodies, such as D-3-hydroxybutyrate and acetoacetate.
Application
3-Hydroxybutyrate Dehydrogenase (3-HBDH) oxidizes selectively (R)-3-hydroxymonocarboxylic acids, or reverse reaction.
Biochem/physiol Actions
3-Hydroxybutyrate dehydrogenase (3-HBDH), a mitochondrial enzyme, catalyzes the reversible oxidation of 3-hydroxybutyrate (3HB) to acetoacetate, with NAD as coenzyme. In mammals, acetyl-coA is metabolized, in one of two pathways, to produce acetoacetate and D-3-hydroxybutyrate, which along with acetone are known as ketone bodies. 3-HBDH reversibly reduces this free acetoacetate to D-3-hydroxybutyrate. In patients with diabetic ketoacidosis (DKA), the ratio of 3HB and acetoacetate can be as high as 10:1, as compared to the normal ratio of 1:1. 3-HBDH enzyme can be used to detect the quantity of 3HB in urine, serum, and blood samples.
Quality
Contaminants: <0.1% LDH, <5% MDH
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Stabilizers: NADH, Ca2+
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
No data available
Flash Point(C)
No data available
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Yoshitaka Nakajima et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 61(Pt 1), 36-38 (2006-03-02)
A recombinant form of D-3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) from Pseudomonas fragi has been crystallized by the hanging-drop method using PEG 3000 as a precipitating agent. The crystals belong to the orthorhombic group P2(1)2(1)2, with unit-cell parameters a = 64.3, b
E B Worrall et al.
The Biochemical journal, 241(1), 297-300 (1987-01-01)
The enzymic determination of D-3-hydroxybutyrate and acetoacetate normally involves the use of 3-hydroxybutyrate dehydrogenase (HBDH, EC 1.1.1.30) of bacterial origin. We show that HBDH from Rhodopseudomonas spheroides (BCL, grade II) contains a 3-hydroxyisobutyrate dehydrogenase (HIBDH) activity: activity with 3-hydroxyisobutyrate as
Fahimeh Khorsand et al.
IET nanobiotechnology, 7(1), 1-6 (2013-05-28)
Precise detection of 3-hydroxybutyrate (HB) in biological samples is of great importance for management of diabetic patients. In this study, an HB biosensor based on single-walled carbon nanotubes (SWCNTs)-modified screen-printed electrode (SPE) was developed to determine the concentration of HB
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