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Merck
  • Solid-phase hybridization efficiency improvement on the magnetic nanoparticle surface by using dextran as molecular arms.

Solid-phase hybridization efficiency improvement on the magnetic nanoparticle surface by using dextran as molecular arms.

Journal of biomedical nanotechnology (2013-09-26)
Zhiyang Li, Haowen Yang, Nongyue He, Wenbiao Liang, Chao Ma, Muhammad Ali A Shah, Yongjun Tang, Song Li, Hongna Liu, Hesheng Jiang, Yafen Guo
PMID24059094
摘要

For reducing the steric hindrance and nonspecific binding of the target DNA, the dextran was used as molecular arms to be immobilized on the surface of magnetic nanoparticles (MNPs). Magnetic separation was used in preparation of dextran-MNPs (DMNPs). Aspartic acid and aminated DNA probe were successively modified on the dextran immobilized on the surface of MNPs. These probe-DMNPs were successfully applied to detect biotin-labeled PCR product of E. coli O157:H7 genome by hybridization. Then the complexes were bonded with streptavidin-modified alkaline phosphatase (ALP-SA). Finally the chemiluminescent signals were detected by adding 3-(2-spiroadamantane)-4-methoxy-4- (3-phosphoryloxy) phenyl-1,2-dioxetane (AMPPD). The results showed that this method had a good specificity, and higher sensitivity than that when only MNPs were used as solid carriers.

材料
货号
品牌
产品描述
31392
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31390
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葡聚糖 来源于明串珠菌 属, Mr ~70,000
31389
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葡聚糖 来源于明串珠菌 属, Mr ~40,000
D5376
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葡聚糖 来源于肠系膜明串珠菌, average mol wt 1,500,000-2,800,000
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葡聚糖 来源于肠系膜明串珠菌, average mol wt 60,000-76,000
D9260
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葡聚糖 来源于肠系膜明串珠菌, average mol wt 9,000-11,000
D1662
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葡聚糖 来源于肠系膜明串珠菌, average mol wt 35,000-45,000
31388
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葡聚糖 来源于明串珠菌 属, Mr ~6,000
00271
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右旋糖酐, analytical standard, for GPC, 25,000
D4876
Sigma-Aldrich
葡聚糖 来源于肠系膜明串珠菌, average mol wt 150,000
00269
Supelco
右旋糖酐, analytical standard, for GPC, 5,000
09184
Sigma-Aldrich
葡聚糖 来源于明串珠菌 属, Mr ~100,000
00268
Supelco
右旋糖酐, analytical standard, for GPC, 1,000
31387
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31397
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31394
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葡聚糖 来源于肠系膜明串珠菌, average mol wt 48,000-90,000
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D4626
Sigma-Aldrich
葡聚糖 来源于肠系膜明串珠菌, average mol wt 15,000-30,000
31425
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31417
Supelco
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00894
Supelco
右旋糖酐, analytical standard, for GPC, 270,000
00893
Supelco
右旋糖酐, analytical standard, for GPC, 150,000
00891
Supelco
右旋糖酐, analytical standard, for GPC, 50,000
00892
Supelco
右旋糖酐, analytical standard, for GPC, 80,000
00895
Supelco
右旋糖酐, analytical standard, for GPC, 410,000
31430
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00270
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右旋糖酐, analytical standard, for GPC, 12,000
31416
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