G005743

HPLC Analysis of Steroids on Ascentis^® Express F5

application for HPLC

属性

• 技術: HPLC: suitable
• 測試參數: column: Ascentis Express F5, 15 cm x 4.6 mm I.D., 2.7 μm particles (53591-U); mobile phase: [A] water; [B] methanol; (50:50, A:B); flow rate: 0.8 mL/min; pressure: 2393 psi (165 bar); column temp.: 35 °C; detector: UV, 240 nm; injection: 5 μL; sample: 10 μg/mL of each in 90: 10, water: methanol
• 適合性: application for HPLC
• 應用: clinical

说明

分析報告

Sample concentrations at 10 ug/mL in 90:10 water:methanol. Acetonitrile does not provide adequate selectivity on this phase. Mass spec was used for peak identification.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

Hydrocortisone
Prednisolone
Prednisone