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Key Documents

817501

TSKgel® Octadecyl-2PW HPLC Column

phase C18 (octadecyl), L × I.D. 15 cm × 6 mm, 5 μm particle size

别名:

TSKgel® Reversed Phase HPLC Column (Polymer-based)

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About This Item

分類程式碼代碼:
41115711
eCl@ss:
32110501

材料

stainless steel hardware

產品線

TSKgel®

製造商/商標名

Tosoh 17501

標籤範圍

1 meq/mL ligand density (estimate)

參數

0-100% organic solvent
1.5 mL/min flow rate
120 bar max. pressure
4-50 °C temperature

技術

HPLC: suitable

長度 × 內徑

15 cm × 6 mm

基質

polymer particle platform

基質活性組

C18 (octadecyl) phase

粒徑

5 μm

孔徑

125 Å

工作pH值

2-12

分離技術

reversed phase

一般說明

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-2PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 125Å pores allow for anaylsis of peptides up to 8,000 Da. Columns packed with larger particle sizes are available for semi-preparative scale separations.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
  • Octadecyl-2PW particles have 125Å pores
  • Octadecyl-4PW particles have 500Å pores
  • Phenyl-5PW RP particles have 1000Å pores
  • Octadecyl-NPR particles are nonporous

外觀

Shipped in 55% acetonitrile - 45% water.

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法律資訊

TSKgel is a registered trademark of Tosoh Corporation

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