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product name
TSK-GEL® 反相 HPLC 色谱柱, phase C18 (octadecyl), L × I.D. 25 cm × 4.6 mm, 5 μm particle size
材料
stainless steel
agency
suitable for USP L1
產品線
TSKgel®
製造商／商標名
Tosoh ODS-80Ts, 17202
標籤範圍
15% Carbon, monolayer, endcapped
參數
1.2 mL/min flow rate
10-100% organic solvent
10-50 °C temperature
300 bar max. pressure
技術
HPLC: suitable
長度 × 內徑
25 cm × 4.6 mm
表面積
425 m2/g
基質
silica particle platform
基質活性組
C18 (octadecyl) phase
粒徑
5 μm
孔徑
100 Å pore diameter
工作pH值
2.0-7.5
分離技術
reversed phase
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一般說明
TSKgel ODS-80Ts is a general purpose column for low MW pharmaceuticals, bases, nucleosides and nucleotides. Common applications include purity checks and peptide mapping. Bonded phase pore size is indicated by the number in the product description, in this case TSKgel ODS-80Ts has 80Å nominal pore size. The pore size of the base silica is 100Å.
應用
TSK gel ODS 80Ts HPLC column may be used for the separation of:
- Tea catechins and quercetin conjugates in blood plasma by high-performance liquid chromatography (HPLC) coupled to electrochemical detection (ECD).
- Active pharmaceutical ingredients (APIs) as well as their impurities in eye drop solution samples by HPLC with photodiode array (PDA) detector.
- Drug substances by LC equipped with ultraviolet-visible (UV-Vis) detector.
TSKgel® ODS (octadecylsilane )-80Ts HPLC Column was used in HPLC analysis, done for the isolation and identification of isoflavones in blood plasma. It was also used in the measurement of quercetin glucosides in onion and in determination of quercetin metabolites in human plasma during HPLC analysis.
外觀
Shipped in 70% methanol - 30% water.
法律資訊
TSKgel is a registered trademark of Tosoh Corporation
Accumulation of quercetin conjugates in blood plasma after the short-term ingestion of onion by women.
Moon JH
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 279 (2), 461-467 (2000)
Accumulation of quercetin conjugates in blood plasma after the short-term ingestion of onion by women
Moon JH, et al.
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 279(2), R461-R467 (2000)
Quality by design: multidimensional exploration of the design space in high performance liquid chromatography method development for better robustness before validation
Monks K, et al.
Journal of Chromatography A, 1232(2), 218-230 (2012)
Daidzein and genistein but not their glucosides are absorbed from the rat stomach.
Mariusz PK
Febs Letters, 447 (2), 287-291 (1999)
Simultaneous determination of twelve tea catechins by high-performance liquid chromatography with electrochemical detection
Sano M, et al.
Analyst, 126(6), 816-820 (2001)
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