ZRB2564

Anti-CYP11B1 Antibody, clone 1J14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 别名: CYPXIB1, Cytochrome P-450c11 (Cytochrome P450C11), Cytochrome P450 11B1, mitochondrial, EC:1.14.15.4, S11BH, Steroid 11-beta-hydroxylase
• 分類程式碼代碼: 12352203

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1J14, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 57.57 kDa; observed mol wt ~52 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: monkey
• 包裝: antibody small pack of 25
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG
• 表位序列: N-terminal half
• Protein ID登錄號: NP_000488
• UniProt登錄號: P15538
• 環保替代類別: Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 基因資訊: human ... CYP11B1(1584)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1J14 is a ZooMAb^® recombinant rabbit monoclonal antibody that detects CYP11B1. It targets an epitope within 11 amino acids from the N-teminal half.

免疫原

KLH-conjugated linear peptide corresponding to 11 amino acids from the N-teminal half of human CYP11B1.

應用

Quality Control Testing

Evaluated by Western Blotting in human adrenal tissue lysate.

Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected CYP11B1 in human adrenal tissue lysate.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound CYP11B1 peptide with at least one hundred thousand-fold (100,000X) higher than with non-specific control peptide.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected CYP11B1 in human adrenal gland tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CYP11B1 in human adrenal gland tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

標靶描述

Cytochrome P450 11B1, mitochondrial (UniProt: P15538; EC:1.14.15.4; EC:1.14.15.4; also known as CYPXIB1, Cytochrome P-450c11 (Cytochrome P450C11), Steroid 11-beta-hydroxylase, CYP11B1) is encoded by the CYP11B1 (also known as S11BH) gene (Gene ID: 1584) in human. CYP11B1 is a member of the cytochrome P450 superfamily of enzymes, which are characterized by their heme-binding domain crucial for their enzymatic activity. CYP11B1 is a mitochondrial enzyme with a structure that includes a signal sequence that directs it to the mitochondria, where it is integrated into the inner mitochondrial membrane. CYP11B1 is primarily involved in the biosynthesis of corticosteroids, specifically in the conversion of 11-deoxycortisol to cortisol in the zona fasciculata of the adrenal cortex. This enzyme′s activity is essential for the production of cortisol, which plays a key role in glucose metabolism, immune function, and stress response. The expression of CYP11B1 is restricted to the adrenal cortex, reflecting its specialized role in steroidogenesis. Its expression and activity are regulated by adrenocorticotropic hormone (ACTH), which is part of the hypothalamic-pituitary-adrenal axis, responding to physiological stress. CYP11B1 is localized to the mitochondria, specifically within the inner membrane. Overactivity of CYP11B1 or mutations that increase its activity can contribute to conditions such as Cushing′s syndrome, characterized by excessive cortisol production and associated symptoms like hypertension, obesity, and glucose intolerance. Conversely, mutations that reduce CYP11B1 activity can lead to adrenal insufficiency, where cortisol production is inadequate, impacting stress response and metabolism. Additionally, genetic variations in CYP11B1 have been implicated in the modulation of blood pressure and may contribute to the development of hypertension. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

重構

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable