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生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1E20, recombinant monoclonal
描述
1E20 Clone
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 48.49 kDa
observed mol wt ~48 kDa
純化經由
using Protein A
物種活性
human
物種活性（以同源性預測）
monkey
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... KRT20(54474)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 1E20 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Cytokeratin-20. It targets an epitope within 23 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of human Cytokeratin-20.
應用
Quality Control Testing
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cytokeratin-20 in HT-29 cell lysate.
Tested applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Cytokeratin-20 in one million HT-29 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cytokeratin-20 in human small intestine tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Cytokeratin-20 peptide with a KD of 6.8 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cytokeratin-20 in HT-29 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cytokeratin-20 in HT-29 cell lysate.
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cytokeratin-20 in HT-29 cell lysate.
Tested applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected Cytokeratin-20 in one million HT-29 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cytokeratin-20 in human small intestine tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Cytokeratin-20 peptide with a KD of 6.8 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cytokeratin-20 in HT-29 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HT-29 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cytokeratin-20 in HT-29 cell lysate.
標靶描述
Keratin, type I cytoskeletal 20 (UniProt: P35900; also known as Cytokeratin-20, CK-20, Keratin-20, K20, Protein IT) is encoded by the KRT20 gene (Gene ID: 54474) in human. Cytokeratin-20 is a heterotetramer of two type I and two type II keratins of the intermediate filament family. It is expressed predominantly in the intestinal epithelium and in luminal cells of colonic mucosa. Its expression is also observed in the Merkel cells of keratinized oral mucosa. It is recognized as a marker of intestinal cell differentiation and is also an excellent marker for certain types of carcinomas, such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Cytokeratin-20 It plays a significant role in maintaining keratin filament organization in intestinal epithelia and when phosphorylated, it also plays a role in the secretion of mucin in the small intestine. It is reported to undergo hyperphosphorylation at serine 13 during the early stages of apoptosis, which becomes less prominent during the later stages. Phosphorylation at serine 13 is shown to increase in response to stress brought on by cell injury. During apoptosis it is cleaved at Asp 228 by caspases. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Moll, R., et al. (1992). Am. J. Pathol. 140(2); 427-447).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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