ZRB1667

Anti-p-DRP1-Ser637 Antibody, clone 5G23 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 分類程式碼代碼: 12352203
• NACRES: NA.43

属性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 5G23, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 81.88 kDa; observed mol wt ~75 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: canine, rat, feline, bovine, mouse, monkey
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; inhibition assay: suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal
• Protein ID登錄號: NP_036192
• UniProt登錄號: O00429
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: phosphorylation (pSer637)
• 基因資訊: human ... DNM1L(10059)

说明

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 5G23 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Dynamin-related protein 1 (DRP1) phosphorylated on serine 637.

免疫原

KLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphoserine 637 from the C-terminal region of human Dynamin-related protein 1 (DRP1).

應用

Quality Control Testing

Evaluated by Western Blotting in a construct containing an N-terminal MBP tagged and C-terminal His tagged recombinant phosphorylated DRP1 (Ser637) protein.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected a construct containing an N-terminal MBP tagged and C-terminal His tagged recombinant phosphorylated DRP1 (Ser637) protein, but did not react with non-phosphorylated DRP1 construct. (DRP1 construct: Courtesy of Dr. Jesse Rinehart, Yale University, School of Medicine)..

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected p-DRP1-Ser637 in lysate from NIH3T3 cells treated with Forskolin (50 mM)..

Peptide Inhibition Assay: Target band detection in a construct containing an N-terminal MBP tagged and C-terminal His tagged recombinant phosphorylated DRP1 (Ser637) protein fragment was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but non the corresponding non-phosphopeptide.

Affinity Binding Assay: A representative lot of this antibody bound p-DRP1-Ser637 with a KD of 1.3 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Dynamin-1-like protein (UniProt: O00429; also known as EC:3.6.5.5, Dnm1p/Vps1p-like protein, DVLP, Dynamin family member proline-rich carboxyl-terminal domain less, Dymple, Dynamin-like protein, Dynamin-like protein 4, Dynamin-like protein IV, HdynIV, Dynamin-related protein 1) is encoded by the DNM1L (also known as DLP1, DRP1) gene (Gene ID: 10059) in human. DRP1 is a ubiquitously expressed, homotetrameric protein that plays an important role in mitochondrial fission during mitosis. DRP1 mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Although DRP1 is essential, but it is not sufficient for mitochondrial fission and requires the recruitment of certain receptors that recruit the enzyme to the outer mitochondrial membrane. In mammals these receptors include MFF, MID49, and MID51. DRP1 phosphorylation/dephosphorylation at two sites near the GED domain (aa 644-735) regulates mitochondrial fission. Its phosphorylation at serine 637 by CaM kinase I and PKA inhibits the GTPase activity that leads to a defect in mitochondrial fission and promotes mitochondrial elongation. Dephosphorylation at this site by protein phosphatase 3 catalytic subunit a (PPP3CA) promotes the fission events. Phosphorylation at serine 616 by Cdk1 and PINK1 activates GTPase and promotes mitochondrial fission. DRP1 is also required for a normal rate of cytochrome c release and caspase activation during apoptosis and this requirement depends upon the cell type and the physiological apoptotic cues. Nine isoforms of DRP1 have been described that are produced by alternative splicing. Isoform 1 with canonical sequence is shown to be brain-specific. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kalia, R., et al. (2018). Nature. 558(7710). 401-405; Fröhlich, C., et al. (2013). EMBO J. 32(9); 1280-1292; Smirnova, E., et al. (2001). Mol. Biol. Cell. 12(8); 2245-2256).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable