推荐产品
生物源
rabbit
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
3J7, recombinant monoclonal
描述
recombinant, expressed in HEK 293 cells
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 106.16 kDa
observed mol wt ~110 kDa
純化經由
using Protein A
物種活性
rat, human
物種活性（以同源性預測）
monkey
包裝
antibody small pack of 25 μL
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
技術
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable
同型
IgG
表位序列
C-terminal
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
phosphorylation (pSer807/pSer811)
基因資訊
human ... RB1(5925)
一般說明
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
特異性
Clone 3J7 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Retinoblastoma-associated protein phosphorylated on serine 807 and 811.
免疫原
KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phosphoserines 807 and 811 from human Retinoblastoma-associated protein (Rb).
應用
Quality Control Testing
Evaluated by Western Blotting in HeLa cells treated with nocodazole.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Rb-Ser807/811 in HeLa cells treated with Nocodazole (40 ng/mL; 8 h).
Tested Applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected phospho-Rb-Ser807/811 in one million serum starved MCF-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Rb-Ser807/811 in C6 cells.
Affinity Binding Assay: A representative lot of this antibody bound phospho-Rb-Ser807-811 peptide with a KD of 9.1 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-Rb-Ser807/811 in human lung cancer and human ovarian cancer tissue sections.
Peptide Inhibition Assay: Target band detection in a lysate from HeLa cells treated with Nocodazole (40 ng/mL; 8 h) was prevented by pre-blocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa cells treated with nocodazole.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-Rb-Ser807/811 in HeLa cells treated with Nocodazole (40 ng/mL; 8 h).
Tested Applications
Flow Cytometry Analysis: 0.1 μg from a representative lot detected phospho-Rb-Ser807/811 in one million serum starved MCF-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Rb-Ser807/811 in C6 cells.
Affinity Binding Assay: A representative lot of this antibody bound phospho-Rb-Ser807-811 peptide with a KD of 9.1 x 10-7 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected phospho-Rb-Ser807/811 in human lung cancer and human ovarian cancer tissue sections.
Peptide Inhibition Assay: Target band detection in a lysate from HeLa cells treated with Nocodazole (40 ng/mL; 8 h) was prevented by pre-blocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
標靶描述
Retinoblastoma-associated protein (UniProt: P06400; also known as p105-Rb, p110-RB1, pRb, Rb, pp110) is encoded by the RB1 gene (Gene ID: 5925) in human. Retinoblastoma (Rb) protein act as a negative regulators of cell cycle and controls the passage of cells from G1 to S phase. It is a key regulator of entry into cell division that acts as a tumor suppressor. It acts as a transcription repressor of E2F1 target genes. In its underphosphorylated, active state it interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Its action is regulated by phosphorylation of several serine and threonine residues on the protein. Phosphorylated Rb protein sequesters transcription factors allowing the transition from G1 to S phase. It is phosphorylated by Cdk6 and Cdk4, and subsequently by Cdk2 at serine 567 in G1, thereby releasing E2F1 which is then able to activate cell growth. It is dephosphorylated in the late M phase. Phosphorylation at threonine 821 and 826 promotes interaction between the C-terminal domain and the pocket domain (aa 373-771), and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Phosphorylation of serine 807 and 811 residues by Cdk3/cyclin-C is shown to be essential for G0-G1 transition. Mutations in Rb1 gene are known to cause childhood retinoblastoma, a malignant tumor that arises from the nuclear layers of the retina. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chinnam, M., and Goodrich, DW. (2011). Curr. Top. Dev. Biol. 94; 129-169; Harbour, JW., et al. (1999). Cell. 98(6); 859-869).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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