推荐产品
生物源
rabbit (recombinant)
品質等級
重組細胞
expressed in HEK 293 cells
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
1E11, recombinant monoclonal
產品線
ZooMAb® learn more
形狀
lyophilized
分子量
calculated mol wt 103.85 kDa
observed mol wt ~103 kDa
物種活性
human, mouse
物種活性（以同源性預測）
rat
包裝
antibody small pack of 25 μL
環保替代產品特色
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
加強驗證
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
技術
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable (peptide)
同型
IgG
UniProt登錄號
環保替代類別
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... ACTN2(88)
一般說明
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
特異性
Clone 1E11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Alpha-Actinin-2. It targets an epitope within 17 amino acids from the N-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to the first 17 amino acids from the N-terminal region of Alpha-actinin-2 from human skeletal muscle.
應用
Anti-alpha Actinin 2, clone 1E11 ZooMAb , Cat. No. ZRB1169, is a highly specific rabbit recombinant monoclonal antibody that targets alpha-Actinin-2 and has been tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected alpha-Actinin-2 in mouse skeletal muscle tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected alpha- Actinin-2 in human skeletal muscle and mouse skeletal muscle tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected alpha-Actinin-2 in human skeletal muscle cells (SkMC).
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected alpha-Actinin-2 in human skeletal muscle frozen tissue sections.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected alpha- Actinin-2 in human skeletal muscle and mouse skeletal muscle tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected alpha-Actinin-2 in human skeletal muscle cells (SkMC).
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected alpha-Actinin-2 in human skeletal muscle frozen tissue sections.
Note: Actual optimal working dilutions must be determined by the end user as specimens and experimental conditions may vary.
標靶描述
Alpha-actinin-2 (UniProt: P35609; also known as Alpha-actinin skeletal muscle isoform 2, F-actin cross-linking protein) is encoded by the ACTN2 gene (Gene ID: 88) in human. Alpha-actinin-2 is expressed in both skeletal and cardiac muscle. It is considered as a bundling protein that acts to crosslink filamentous actin molecules and titin molecules from adjoining sarcomeres at Z-discs, a function that is modulated by phospholipids. It has a rod-shaped structure and it homodimerizes in an anti-parallel fashion. Each monomer contains an N-terminal actin-binding region composed of two calponin homology domains (aa 38-142 and 151-257), two C-terminal EF hand domains (aa 753-788 and 789-824), and four tandem spectrin-like repeats (aa 281-391, 401-506, 516-627, and 637-740). It has also been shown to localize to dendritic spines, enriched within the post-synaptic density (PSD). In rat hippocampal neurons loss of alpha-actinin-2 is shown to create an increased density of immature, filopodia-like protrusions that fail to mature into a mushroom-shaped spine during development and its knockdown prevents the recruitment and stabilization of PSD in the spine, resulting in failure of synapse formation. Mutations in ACTN2 gene is known to cause cardiomyopathy with ventricular hypertrophy leading to dyspnea, syncope, collapse, palpitations, and chest pain. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hodges, JL, et al. (2014). PLoS ONE 9(7): e101770).
外觀
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose. Normal appearance is a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
重構
30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
儲存和穩定性
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
法律資訊
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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