推荐产品
生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~50 kDa
物種活性
rat, human, mouse
濃度
~1.0 mg/mL
技術
immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 cells
western blot: 0.5-1 μg/mL using PC12 cell lysates
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... YBX1(4904)
mouse ... Ybx1(22608)
rat ... Ybx1(500538)
一般說明
Y-box-结合蛋白（YB-1）也称为核酸酶敏感元件结合蛋白1（NSEP1），是包含冷休克结构域（CSD）的蛋白质的成员。它由三个结构域组成：N末端被认为是反式激活调节结构域，高度保守的核酸结合结构域（CSD）和C末端结构域，参与蛋白质-蛋白质相互作用。
特異性
抗YB-1（C末端）抗体对大鼠、小鼠和人类的YB-1具有特异性。该免疫肽特异性抑制免疫印迹法检测YB-1条带。
免疫原
合成肽对应于与KLH偶联的人YB-1氨基酸307-324。在大鼠和小鼠YB-1蛋白中的相应序列是相同的。
應用
抗YB-1（C末端）抗体已用于
- 免疫印迹
- 免疫沉淀
- 免疫荧光
- 双重免疫染色
生化／生理作用
Y-box结合蛋白（YB-1）显示与Y-box启动子元件结合以调节基因表达。它在多种细胞功能中起着重要作用，例如转录和翻译调节，DNA修复，耐药性和应激反应。
外觀
溶于含有15 mM叠氮化钠的0.01M磷酸盐缓冲盐水（pH 7.4）溶液中。
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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儲存類別代碼
12 - Non Combustible Liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Kimitoshi Kohno et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 25(7), 691-698 (2003-06-20)
The Y-box-binding protein (YB-1) represents the most evolutionary conserved nucleic-acid-binding protein currently known. YB-1 is a member of the cold-shock domain (CSD) protein superfamily. It performs a wide variety of cellular functions, including transcriptional regulation, translational regulation, DNA repair, drug
Daniel M Breitkopf et al.
Kidney international, 97(2), 289-303 (2019-12-29)
Systemic lupus erythematosus (SLE) is an autoimmune disease and lupus nephritis is a major risk factor for morbidity and mortality. Notch-3 signaling induced by membrane-bound or soluble ligands such as YB-1 constitutes an evolutionarily conserved pathway that determines major decisions
K Matsumoto et al.
Trends in cell biology, 8(8), 318-323 (1998-08-15)
Y-box proteins are multifunctional regulators of gene expression. In somatic cells, they have the capacity to exert positive and negative effects on both transcription and translation. In Xenopus oocytes, they help to mask maternal mRNA and couple the transcription of
YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts
Wei W J, et al.
Nucleic Acids Research, 40(17), 8622-8636 (2012)
N V Bobkova et al.
PloS one, 10(9), e0138867-e0138867 (2015-09-24)
The Y-box binding protein 1 (YB-1) is a member of the family of DNA- and RNA binding proteins. It is involved in a wide variety of DNA/RNA-dependent events including cell proliferation and differentiation, stress response, and malignant cell transformation. Previously
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