XNACRL

Extract-N-Amp^™ Cellular RNA Lysis Buffer

Optimized for rapid cell lysis and RNA stabilization

• 别名: RNA extraction buffer, RNA isolation buffer, RNA lysis buffer
• 分類程式碼代碼: 41106306

属性

• 品質等級: 200
• 形狀: liquid
• 儲存條件: OK to freeze (-10 to-25°C)
• 適合性: suitable for PCR (suitable for RT-qPCR)
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

一般說明

The Extract-N-AmpTM Cellular RNA Lysis buffer (XNACRL) is a single working solution that allows for rapid, room-temperature extraction and stabilization of RNA from a range of cells without heat, agitation or clean up. This is a simple and robust method for preparing cell lysate and stabilizing the extracted RNA for gene expression analysis directly from the cell lysate without the need for purification step prior to analysis.

應用

• The Extract-N-AmpTM Cellular RNA Lysis buffer is suitable for direct use with
• qRT-PCR
• RT-LAMP
• NGS library preparation

特點和優勢

• From cell lysis to qRT-PCR in 15 minutes
• High Throughput and automation-friendly
• No RNA purification or clean up necessary
• No columns, heat, or centrifugation necessary
• Compatible with both adherent and non-adherent mammalian cell types
• Sensitivity range: 10-100k cells •May be paired with DNase I to ensure removal of DNA for more sensitive processes

外觀

Liquid

儲存和穩定性

Freezer (-10 to-25°C)

法律資訊

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

• DNA-free JumpStart Taq DNA Polymerase 250U, 1.5KU
• DNA-free JumpStart Taq DNA Polymerase