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Merck

TRUERGD

Sigma-Aldrich

TrueGel3D RGD

Integrin Adhesion Peptide

别名:

3D cell culture matrix

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

TrueGel3D®是各种不同种类的商业化聚合物支架,用于体外肿瘤模型的三维培养。水凝胶中的RGD肽用于细胞粘附。
细胞外基质蛋白(纤连蛋白,玻连蛋白)上的 RGD 序列促进细胞与细胞整联蛋白受体的粘附。TrueGel3D® RGD 肽 N 端的巯基可作为将 RGD 基序与聚合物(Dextran/PVA)结合的连接子。聚合物上缀合的 RGD 基序可促进细胞与适当整联蛋白受体的粘附。

應用

具有细胞粘附基序(RGD)的聚合物用于产生细胞粘附水凝胶

特點和優勢

包含细胞粘附基序 RGD(乙酰基-Cys-Doa * -Doa-Gly-Arg-Gly-Asp-Ser-Pro-NH2),可与细胞上适当的整联蛋白受体结合。

*Doa:8-氨基-3,6-二氧杂辛酸

成分

TRUERGD 包含 RGD 可降解聚合物(冻干的和水)

法律資訊

TRUEGEL3D is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10 - Combustible liquids


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L P Ferreira et al.
Acta biomaterialia, 75, 11-34 (2018-05-29)
Three-dimensional multicellular tumor models are receiving an ever-growing focus as preclinical drug-screening platforms due to their potential to recapitulate major physiological features of human tumors in vitro. In line with this momentum, the technologies for assembly of 3D microtumors are

商品

TrueGel3D™ synthetic hydrogel can be used to study co-culture of tumor and stromal cells.

Fibroblasts that are present in the stroma secrete glycoproteins and polysaccharides to form extracellular matrix. They maintain the structural integrity within the connective tissue and play a key role in cancer, chronic obstructive pulmonary diseases, idiopathic pulmonary fibrosis, scleroderma and several other diseases.

TrueGel3D synthetic hydrogel can be used to study cyst formation of MDCK cells.

TrueGel3D hydrogels kits for 3D cell culture troubleshooting guide

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实验方案

TrueGel3D™ is a chemically-defined hydrogel formed by mixing polymers with crosslinkers. This hydrogel permits the encapsulation of cells to put them in a three-dimensional environment to better mimic the native tissue environment.

TrueGel3D™ is chemically defined hydrogel, enables cell growth in 3D environment and mimics native tissue environment. Slow gelation facilitates filling of microchannels or syringes. Find detail protocol of slow gelation for TrueGel3D™ kits

相关内容

An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation

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