推荐产品
形狀
buffered powder
品質
Microbiologically tested.
一般說明
胰蛋白胨组分是一种蛋白胨（氨基酸和短肽的混合物），来源于乳蛋白酪蛋白的混合酶水解物（胰酶）。
應用
胰蛋白示磷酸盐培养基可用作以下物质的组成成分：
- 用于培养BME26蜱胚细胞系的Leibovitz L-15培养基
- 用于制备鸡胚纤维母细胞(CEF)的M199培养基
- 用于转染前培养幼仓鼠肾(BHK-21)的Glasgow′极限必需培养基(GMEM)
除了用于苛养菌的生长外，还研究了胰蛋白胨磷酸盐肉汤（TPB）作为补充剂，用于制备能够支持在高密度灌注培养搅拌槽生物反应器中BHK-21细胞的疫苗生产以及SF21昆虫细胞生长的培养基。
生化／生理作用
胰蛋白胨磷酸盐肉汤含有氨基酸类营养素和存活因子，能够支持布鲁氏菌（Brucella）、链球菌（Streptococcus）和奈瑟氏球菌（Neisseria）等苛养菌的生长，以及昆虫和动物细胞等真核细胞的生长。葡萄糖提供可发酵的碳水化合物，以供苛养菌使用。氯化钠保持渗透和离子平衡，磷酸二钠提供基本的缓冲能力。
成分
胰蛋白胨磷酸盐肉汤（TPB）由四种成分组成：胰蛋白胨（20g/L），葡萄糖（2g/L），NaCl（5g/L）以及磷酸二钠（2.5g/L），通常调节至pH 7.3。胰蛋白胨组分是一种蛋白胨（氨基酸和短肽的混合物），来源于乳蛋白酪蛋白的混合酶水解物（胰酶）。该水解物含有氨基酸类营养素和存活因子，能够支持布鲁氏菌、链球菌和奈瑟氏球菌等苛养菌的生长，以及昆虫和动物细胞等真核细胞的生长。葡萄糖提供可发酵的碳水化合物，以供苛养菌使用。氯化钠保持渗透和离子平衡，磷酸二钠提供基本的缓冲能力。
也與該產品經常一起購買
产品编号
说明
价格
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
The use of sonicated lipid vesicles for mass spectrometry of membrane protein complexes
Chorev DS, et al.
Nature Protocols, 15(5), 1690-1706 (2020)
Dror S Chorev et al.
Nature protocols, 15(5), 1690-1706 (2020-04-03)
Recent applications of mass spectrometry (MS) to study membrane protein complexes are yielding valuable insights into the binding of lipids and their structural and functional roles. To date, most native MS experiments with membrane proteins are based on detergent solubilization.
S N Saha et al.
Vaccine, 7(4), 357-363 (1989-08-01)
Studies were undertaken to develop a cheaper medium with indigenous sources of peptone and casein hydrolysate for continuous culture of BHK-21 (suspension) cells and production of foot-and-mouth disease (FMD) vaccine. Eleven batches of experimental media were prepared using different indigenous
A I Josemans et al.
Annals of the New York Academy of Sciences, 969, 141-146 (2002-10-17)
The in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants, was first achieved in 1985. Culture media were usually supplemented with serum and tryptose phosphate broth, both undefined components, contributing to great variability. Recently, we
S M Deutschmann et al.
Enzyme and microbial technology, 16(6), 506-512 (1994-06-01)
Spodoptera frugiperda insect cells (IPLB-Sf21-AE) (Sf21), infected with baculovirus expression vectors during their exponential growth phase, are commonly used to produce a variety of heterologous recombinant proteins. In the present study the culture conditions of these insect cells were studied
商品
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门