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品質等級
等級
BioPerformance Certified
化驗
≥99% (titration)
形狀
crystalline powder
儲存條件
dry at room temperature
技術
cell culture | mammalian: suitable
雜質
endotoxin and total aerobic microbial count, tested
顏色
white
有用的pH值範圍
7.4-8.8
pKa (25 °C)
8.1
mp
187 °C
正離子痕跡
heavy metals (as Pb): ≤5 ppm
吸收
≤0.02 at 290 at 20%
應用
diagnostic assay manufacturing
general analytical
life science and biopharma
sample preparation
SMILES 字串
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChI 密鑰
SEQKRHFRPICQDD-UHFFFAOYSA-N
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一般說明
三（羟甲基）甲基甘氨酸是一种带电离子的生物缓冲剂，通常用于酶测定、电泳和细胞培养研究。与甘氨酸相比，它具有更高的负电荷，这使得它能够更快地迁移。三（羟甲基）甲基甘氨酸′的高离子强度能够引起更多的离子迁移和更少的蛋白质迁移，从而在低丙烯酰胺凝胶中分离低分子量蛋白质。已经观察到，通过电泳，三（羟甲基）甲基甘氨酸在1至100 kDa蛋白质分离中的有效性。根据一项研究，在使用萤火虫荧光素酶进行ATP测定时，25 mmol/L的三（羟甲基）甲基甘氨酸缓冲液是经过测试的其他十种缓冲液中表现最有效。此外，三（羟甲基）甲基甘氨酸还可用于毛细管区带电泳、高效液相色谱和离子交换色谱。
應用
三（羟甲基）甲基甘氨酸作为一种多功能缓冲剂，适用于在低百分比的丙烯酰胺凝胶中分离低分子量的蛋白质，已经证明使用萤火虫荧光素酶进行ATP测定的有效性，并在研究辐射引起的膜损伤时表现出清除羟自由基的潜力。此外，它在分离和纯化参与特定mRNA翻译并含有特定肽-tRNA分子的核糖体中发挥了作用。.三（羟甲基）甲基甘氨酸还被用于合成RNA寡核苷酸和制备高密度DNA微阵列，从而能够通过光刻术法探索核酸·配体相互作用和文库构建的机制。
用于分离低分子量的肽的缓冲剂组分。
特點和優勢
- 适用作为缓冲剂组分，用于电泳和蛋白质分离
- 有效缓冲pH 7.4-8.8（25 °C），pKa为8.1（25 °C）
- 测试内毒素和总有氧微生物计数
- 测试以确认低水平的重金属污染，确保各种应用的适用性
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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