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Merck

T4319

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA

peroxidase substrate

别名:

TMB

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

品質等級

形狀

liquid

運輸包裝

wet ice

儲存溫度

2-8°C

相关类别

應用

3,3′,5,5′-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA has been used as a substrate for anti-bovine IgG-peroxidase conjugated antibody and for horse radish peroxidase-conjugated mouse anti-mouse IgG in Poly (ADP-ribose) binding assays.
Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 25% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Efficient single-strand break repair requires binding to both poly (ADP-Ribose) and DNA by the central BRCT domain of XRCC1
Polo LM, et al.
Testing, 26(3), 573-581 (2019)
Protection of cattle against a natural infection of Fasciola hepatica by vaccination with recombinant cathepsin L1 (rFhCL1)
Golden O, et al.
Vaccine, 28(34), 5551-5557 (2010)
Luis M Polo et al.
Cell reports, 26(3), 573-581 (2019-01-17)
XRCC1 accelerates repair of DNA single-strand breaks by acting as a scaffold protein for the recruitment of Polβ, LigIIIα, and end-processing factors, such as PNKP and APTX. XRCC1 itself is recruited to DNA damage through interaction of its central BRCT

商品

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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