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T3405

Sigma-Aldrich

3,3′,5,5′-四甲基联苯胺 二盐酸盐

chromogenic, tablet

别名:

4,4′-二氨基-3,3′,5,5′-四甲基联苯 二盐酸盐

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About This Item

经验公式（希尔记法）:
C16H20N2 · 2HCl
CAS号:
64285-73-0
分子量:
313.27
EC號碼:
264-769-6
MDL號碼:
MFCD00012961
分類程式碼代碼:
12352204
PubChem物質ID:
24900140
NACRES:
NA.83

产品名称

3,3′,5,5′-四甲基联苯胺 二盐酸盐, tablet, 1 mg substrate per tablet

品質等級

200

形狀

tablet

mp

≥300 °C

儲存溫度

2-8°C

SMILES 字串

Cl[H].Cl[H].Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H

InChI 密鑰

NYNRGZULARUZCC-UHFFFAOYSA-N

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應用

3,3′,5,5′-四甲基联苯胺二盐酸已作为底物用于
  • 小鼠血清ELISA检测中的小鼠IgG（γ特异性）辣根过氧化物酶偶联抗体
  • 初始和效应T细胞裂解物的蛋白酪氨酸磷酸酶检测中的小鼠过氧化物酶抗体
  • 糖尿病患者血清样本的氢过氧化物酶法(EH)检测和氧化/抗氧化平衡(PAB)检测

3,3′,5,5′-四甲基联苯胺 (TMB) 是一种适用于酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物，该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应（变成黄色），并在 450nm 处读取数据。

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
SLCH0851
SLCC7811
SLBS2228V
SLBQ4513V
SLBK7054V

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Yan-Qin Huang et al.
Analytical and bioanalytical chemistry, 410(28), 7385-7394 (2018-09-15)
We certify that protamine-gold nanoclusters (PRT-AuNCs) synthesized by one-pot method exhibit peroxidase-like activity. The catalytic activity of PRT-AuNCs followed typical Michaelis-Menten kinetics and exhibited higher affinity to 3,3',5,5'-tetramethylbenzidine (TMB) as the substrate compared to that of natural horseradish peroxidase. Meanwhile
Beth C Holbrook et al.
Virology, 476, 124-133 (2014-12-30)
Respiratory infection of young infants results in increased morbidity and mortality compared to infection of adults. In spite of the significance of this health issue, our understanding of the immune response elicited in infants especially in the respiratory tract is
Targeting phospho-Ser422 by active Tau Immunotherapy in the THYTau22 mouse model: a suitable therapeutic approach
Troquier L, et al.
Current Alzheimer Research, 9(4), 397-405 (2012)
Darcy S O Mora et al.
Fish & shellfish immunology, 71, 255-263 (2017-09-04)
An experimental contraceptive vaccine was evaluated in Atlantic salmon (Salmo salar). A peptide derived from the beta subunit of luteinizing hormone (LH) was conjugated to two different carrier proteins, bovine serum albumin (BSA) and keyhole limpet hemocyanin (KLH), and formulated
Thiru Vanniasinkam et al.
Methods in molecular biology (Clifton, N.J.), 1785, 121-128 (2018-05-02)
This chapter describes a strategy for mapping linear B-cell epitopes of proteins using synthetic biotinylated peptides in an ELISA.A set of overlapping peptides were designed based upon a known amino acid sequence of the target protein, VapA (Virulence-associated Protein A)
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商品

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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