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Merck
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Key Documents

STRN50

Sigma-Aldrich

Spectrum™ 植物总RNA试剂盒

greener alternative

sufficient for 50 purifications

别名:

Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit

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About This Item

分類程式碼代碼:
41105501
NACRES:
NA.52

用途

sufficient for 50 purifications

品質等級

200

環保替代產品特色

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

技術

RNA purification: suitable

環保替代類別

Aligned,

儲存溫度

15-25°C

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相关类别

一般說明

Spectrum植物总RNA试剂盒利用了裂解液、结合化学反应法以及方便的柱式“结合-洗涤-洗脱”形式，可在大约30分钟内从100 mg组织中纯化出多达100 μg总RNA。典型得率范围为20–60 μg。

在液氮中将组织研磨成细粉末之后，细胞裂解，细胞残渣通过物理和化学方法与内源性RNA分离。随后，RNA结合到柱式支撑硅胶底物上，经过几遍洗涤去除污染物。

从柱上洗脱总RNA，然后在Northern杂交、RT-PCR和qRT-PCR等常规应用中使用。
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。本产品含有本安型化学制剂。

應用

光谱® 植物总RNA试剂盒已被用于从培养的棉纤维的地纤维组织、印度楝组织、以及 瓶子草（一种食虫植物）中分离RNA。

特點和優勢

  • 每次制备至多可获得60 μg高纯度浓缩RNA
  • 实验方案高度有效，可在30分钟内完成RNA纯化
  • 专为研究困难的植物组织而设计

法律資訊

SabreLite is a registered trademark of Pelican Products, Inc.
Spectrum is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

標靶器官

Liver,Heart

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

分析证书(COA)

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S B Gould et al.
Proceedings. Biological sciences, 286(1909), 20191491-20191491 (2019-08-23)
Pyruvate : ferredoxin oxidoreductase (PFO) and iron only hydrogenase ([Fe]-HYD) are common enzymes among eukaryotic microbes that inhabit anaerobic niches. Their function is to maintain redox balance by donating electrons from food oxidation via ferredoxin (Fd) to protons, generating H2
Emir Islamovic et al.
Molecular plant-microbe interactions : MPMI, 28(1), 42-54 (2014-09-17)
Ustilago maydis, causal agent of corn smut disease, is a dimorphic fungus alternating between a saprobic budding haploid and an obligate pathogenic filamentous dikaryon. Maize responds to U. maydis colonization by producing tumorous structures, and only within these does the
Transcriptome analysis of sarracenia, an insectivorous plant.
Srivastava A
DNA Research : An International Journal for Rapid Publication of Reports on Genes and Genomes, 18(4), 253-261 (2011)
Asfaw Degu et al.
BMC plant biology, 19(1), 69-69 (2019-02-13)
Grape leaves provide the biochemical substrates for berry development. Thus, understanding the regulation of grapevine leaf metabolism can aid in discerning processes fundamental to fruit development and berry quality. Here, the temporal alterations in leaf metabolism in Merlot grapevine grown
Erin M Rehrig et al.
Frontiers in plant science, 5, 407-407 (2014-09-06)
Plant responses to insects and wounding involve substantial transcriptional reprogramming that integrates hormonal, metabolic, and physiological events. The ability to respond differentially to various stresses, including wounding, generally involves hormone signaling and trans-acting regulatory factors. Evidence of the importance of
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商品

Genomic DNA Sample Purification and Quality Assessment

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

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