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生物源
mouse
重組細胞
expressed in E. coli
化驗
≥98% (HPLC)
≥98% (SDS-PAGE)
形狀
lyophilized
效力
8-10 ng/mL ED50
分子量
28.7 kDa
包裝
pkg of 10 μg
技術
cell culture | mammalian: suitable
雜質
<0.1 EU/μg endotoxin, tested
顏色
white to off-white
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
mouse ... PDGFA(18590)
一般說明
The PDGFs are stored in platelet α-granules and are released upon platelet activation. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids). Recombinant murine PDGF-AA is a 28.7 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
應用
PDGF-AA from mouse has been used as a supplement in the media used for differentiation of neurospheres.
生化／生理作用
The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-AA is crucial for optimal wound healing.
序列
MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT
外觀
Lyophilized with no additives.
重構
Centrifuge the vial prior to opening. Reconstitute in 10 mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
Interleukin-1 mitogenic activity for fibroblasts and smooth muscle cells is due to PDGF-AA.
Raines EW
Science, 243, 393-396 (1989)
Isolation of neural stem cells from the postnatal cerebellum.
Lee A
Nature Neuroscience, 8, 723-729 (2005)
Detection of platelet-derived growth factor (PDGF)-AA in actively healing human wounds treated with recombinant PDGF-BB and absence of PDGF in chronic nonhealing wounds.
Pierce GF
The Journal of Clinical Investigation, 96, 1336-1350 (1995)
Native and oxidized LDL enhances production of PDGF AA and the surface expression of PDGF receptors in cultured human smooth muscle cells.
Stiko-Rahm A
Arteriosclerosis and Thrombosis, 12, 1099-1109 (1992)
Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities.
Leveen P
Genes & Development, 8, 1875-1887 (1994)
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