推荐产品
生物源
alpaca
品質等級
重組細胞
expressed in E. coli
共軛
unconjugated
抗體表格
affinity purified VHH antibody
無性繁殖
RP55, monoclonal
化驗
>95% (SDS-PAGE)
形狀
lyophilized solid
分子量
14.3 kDa
~15-18 kDa (SDS PAGE under reducing condition)
純化經由
immobilized metal affinity chromatography (IMAC)
濃度
1 mg/mL
技術
ELISA: 1:2000-1:10000
immunofluorescence: 1:500-1:2000
western blot: 1:1000-1:5000
顏色
colorless
pH值
( in PBS, pH 7.4)
同型
VHH
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
一般說明
mCherry is a monomeric red fluorescent protein (mRFP) belonging to the mFruits family which is brighter and more photostable compared to the first-generation mRFP1, making them ideal for fluorescence microscopy. mCherry has an excitation maximum at 587 nm and an emission maximum at 610 nm. mCherry protein was derived from DsRed, a red fluorescent protein from the coral Discosoma (disc anemone). mCherry is 236 amino acids (aa) in length with a theoretical molecular weight of 28 kDa and has a crystal structure with the chromophore forming a central helix shielded within an eleven-stranded beta-barrel. Alpaca derived anti-mCherry VHH single domain antibody (molecular weight: 14.3 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
特異性
mCherry
免疫原
Recombinant full length mCherry protein
應用
ELISA, WB, IF
包裝
50 µg per vial / 100 µg per vial
外觀
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.11
重構
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
儲存和穩定性
Stable for 1 year at 4°C from date of receipt.
其他說明
Storage at 4°C; Do not freeze.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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