推荐产品
生物源
alpaca
品質等級
重組細胞
expressed in E. coli
共軛
unconjugated
抗體表格
affinity purified VHH antibody
無性繁殖
GE10, monoclonal
化驗
>95% (SDS-PAGE)
形狀
lyophilized solid
分子量
13.7 kDa
~15-18 kDa (SDS PAGE under reducing condition)
純化經由
immobilized metal affinity chromatography (IMAC)
物種活性
SARS coronavirus
濃度
1 mg/mL
技術
ELISA: 1:2000-1:10000
immunofluorescence: 1:100-1:600
immunoprecipitation (IP): 0.5-4.0 μg
western blot: 1:1000-1:5000
顏色
colorless
pH值
( in PBS, pH 7.4)
同型
VHH
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
一般說明
Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488 nm. Alpaca derived anti-GFP VHH single-domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility. Alpaca derived anti-GFP VHH single-domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility. Alpaca derived anti-GFP VHH single-domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components.
特異性
Green fluorescence protein (GFP) and other derivatives of GFP
免疫原
Recombinant full length GFP protein
應用
ELISA, WB, IP, IF
包裝
50 µg per vial / 100 µg per vial
外觀
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.4
重構
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
儲存和穩定性
Stable for 1 year at 4°C from date of receipt.
其他說明
Storage at 4°C; Do not freeze.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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